Standard Curve (Sample)
Human Eotaxin ELISA Kit | CCL11 elisa kit
Eotaxin Human ELISA Kit
Gene Names
CCL11; SCYA11
Reactivity
Human
Applications
ELISA
Synonyms
Eotaxin; N/A; Eotaxin Human ELISA Kit; Human Eotaxin; CCL11 elisa kit
Reactivity
Human
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 2pg/ml
Applicable Applications for CCL11 elisa kit
ELISA
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CCL11 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Eotaxin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Eotaxin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Eotaxin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Eotaxin can be calculated.
Background: Eotaxin, also known as Chemokine (C-C motif) ligand 11 (CCL11), is a small cytokine belonging to the CC chemokine family. These family members play an important role in the recruitment of leukocytes to inflammatory lesions. Eotaxin is a potent inducer of eosinophil chemotaxis leading to eosinophil migration in vitro and accumulation in vivo. It also interacts with CCR2 and CCR5 and can thus affect the responses of monocytes, which express both receptors. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. It has been found that eotaxin has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3.
Background: Eotaxin, also known as Chemokine (C-C motif) ligand 11 (CCL11), is a small cytokine belonging to the CC chemokine family. These family members play an important role in the recruitment of leukocytes to inflammatory lesions. Eotaxin is a potent inducer of eosinophil chemotaxis leading to eosinophil migration in vitro and accumulation in vivo. It also interacts with CCR2 and CCR5 and can thus affect the responses of monocytes, which express both receptors. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. It has been found that eotaxin has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,732 Da
NCBI Official Full Name
eotaxin
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 11
NCBI Official Symbol
CCL11
NCBI Official Synonym Symbols
SCYA11
NCBI Protein Information
eotaxin; eotaxin-1; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
UniProt Protein Name
Eotaxin
UniProt Gene Name
CCL11
UniProt Synonym Gene Names
SCYA11
UniProt Entry Name
CCL11_HUMAN
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Product Notes
The Human CCL11 ccl11 (Catalog #AAA58999) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA58999 ELISA Kit recognizes Human CCL11. AAA Biotech's Eotaxin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CCL11 ccl11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eotaxin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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