Standard Curve (Sample)
Human Ephrin B2 (EFNB2) ELISA Kit | EFNB2 elisa kit
Human Ephrin B2 ELISA Kit (EFNB2)
Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Reactivity
Human
Synonyms
Ephrin B2 (EFNB2); N/A; Human Ephrin B2 ELISA Kit (EFNB2); EFNB2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of EFNB2. No significant cross-reactivity or interference between EFNB2 and analogues was observed.
Sequence Length
333
Samples
Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Sensitivity
< 0.061 ng/mL
Intra-assay Precision
3 samples with low, middle and high level EFNB2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
3 samples with low, middle and high level EFNB2 were tested on 3 different plates, 8 replicates in each plate. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for EFNB2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for EFNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFNB2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for EFNB2 is added to the wells and binds to the combination of capture antibody-EFNB2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of EFNB2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven EFNB2 standard dilutions and EFNB2 sample concentration determined.
Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EFNB2 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EFNB2 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,923 Da
NCBI Official Full Name
ephrin-B2
NCBI Official Synonym Full Names
ephrin B2
NCBI Official Symbol
EFNB2
NCBI Official Synonym Symbols
HTKL; EPLG5; Htk-L; LERK5
NCBI Protein Information
ephrin-B2
UniProt Protein Name
Ephrin-B2
UniProt Gene Name
EFNB2
UniProt Synonym Gene Names
EPLG5; HTKL; LERK5; LERK-5; HTK-L
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Product Notes
The Human EFNB2 efnb2 (Catalog #AAA281416) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281416 ELISA Kit recognizes Human EFNB2. It is sometimes possible for the material contained within the vial of "Ephrin B2 (EFNB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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