Human F box/WD repeat containing protein 7 (FBXW7) ELISA Kit | FBXW7 elisa kit

Human F box/WD repeat containing protein 7 (FBXW7) ELISA Kit

Cat. Num. AAA246453

• Gene Names: FBXW7; AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10
• Reactivity: Human
• Synonyms: F box/WD repeat containing protein 7 (FBXW7); N/A; Human F box/WD repeat containing protein 7 (FBXW7) ELISA Kit; FBXW7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FBXW7. No significant cross-reactivity or interference between FBXW7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBXW7 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for FBXW7 elisa kit

Principle of the Assay: FBXW7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FBXW7 antibody and an FBXW7-HRP conjugate. The assay sample and buffer are incubated together with FBXW7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXW7 concentration since FBXW7 from samples and FBXW7-HRP conjugate compete for the anti-FBXW7 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXW7 from the sample, fewer sites are left to bind FBXW7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXW7 concentration in each sample is interpolated from this standard curve.

Intended Uses: This FBXW7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FBXW7. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for FBXW7 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 44887885
• NCBI GeneID: 55294
• Molecular Weight: 66,120 Da
• NCBI Official Full Name: F-box/WD repeat-containing protein 7
• NCBI Official Synonym Full Names: F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
• NCBI Official Symbol: FBXW7
• NCBI Official Synonym Symbols: AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10
• NCBI Protein Information: F-box/WD repeat-containing protein 7; archipelago; F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; homolog of C elegans sel-10; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
• UniProt Protein Name: F-box/WD repeat-containing protein 7
• UniProt Gene Name: FBXW7
• UniProt Synonym Gene Names: FBW7; FBX30; SEL10; hAgo
• UniProt Entry Name: FBXW7_HUMAN