Standard Curve (Sample)
Human FGF-Basic ELISA Kit | FGF-Basic elisa kit
FGF-Basic (Human) OmniKine ELISA Kit
Gene Names
FGF2; BFGF; FGFB; FGF-2; HBGF-2
Reactivity
Human
Synonyms
FGF-Basic; N/A; FGF-Basic (Human) OmniKine ELISA Kit; Heparin-binding growth factor 2; HBGF-2; Basic fibroblast growth factor; bFGF; FGFB; FGF-Basic elisa kit
Reactivity
Human
Specificity
The OmniKine™ Human FGF-Basic ELISA is capable of recognizing both recombinant and naturally produced Human FGF-Basic proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
Form/Format
12 x 8-Well Microstrips
Sequence Length
288
Assay Type
Quantitative Sandwich
Samples
Supernatants, Sera and Plasma
Sensitivity
63-4000 pg/ml
Detection Method
Colorimetric 450 nm
Subtype
None
Preparation and Storage
Store entire kit at 4 degree C until use.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FGF-Basic elisa kit
Background: Human FGF-Basic or Fibroblast Growth Factor 2, also known commonly as HBGF-2 or Heparin-Binding Growth Factor 2, is a 288 amino acid cytokine protein encoded by the FGF2 gene at locus 4q26 on chromosome 4. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, would healing and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. The monomeric FGF-Basic protein interacts with many other proteins such as CSPG4 and FGFBP1 while also believed to be found in a complex with FGFBP1, FGF1 and FGF2. Expression patterns for this protein typically take place in granulosa and cumulus cells, but FGF2 is also found in hepatocellular carcinoma cells (not non-cancerous liver tissue). Several experiments were performed on FGF-Basic to determine its pH and temperature properties. It has been recently determined that FGF-Basic retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degree C, while being inactivated after 3 minutes at 60 degree C or 1 minute at 80 degree C.
Principle of the Assay: The OmniKine™ Human FGF-Basic ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human FGF-Basic concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" EnzymeLinked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human FGF-Basic while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Principle of the Assay: The OmniKine™ Human FGF-Basic ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human FGF-Basic concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" EnzymeLinked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human FGF-Basic while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,203 Da
NCBI Official Full Name
fibroblast growth factor 2 34 kDa isoform
NCBI Official Synonym Full Names
fibroblast growth factor 2
NCBI Official Symbol
FGF2
NCBI Official Synonym Symbols
BFGF; FGFB; FGF-2; HBGF-2
NCBI Protein Information
fibroblast growth factor 2
UniProt Protein Name
Fibroblast growth factor 2
UniProt Gene Name
FGF2
UniProt Synonym Gene Names
FGFB; FGF-2; bFGF; HBGF-2
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Product Notes
The Human FGF-Basic fgf2 (Catalog #AAA315608) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA315608 ELISA Kit recognizes Human FGF-Basic. It is sometimes possible for the material contained within the vial of "FGF-Basic, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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