Standard Curve (Sample)
(Human FGF1 PicoKine ELISA Kit standard curve)
Human FGF1 ELISA Kit | FGF1 elisa kit
Human FGF1 PicoKine ELISA Kit
Gene Names
FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
Reactivity
Human
Synonyms
FGF1; N/A; Human FGF1 PicoKine ELISA Kit; aFGF; FGF 1; FGF-1; Fgf-1; Fgfa; FGFA; HBGF1; HBGF-1; P05230; fgf19; Fibroblast growth factor 1; fibroblast growth factor 1 (acidic); FGF1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human FGF1
Sequence Length
155
Assay Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Anticoagulant
heparin or EDTA
TMB incubation time
15-20
Immunogen
E Coli, A2-D155
Sample Data
Concentration(pg/ml): |0|31.2|62.5|125|250|500|1000|2000
O.D.: |0.007|0.069|0.161|0.359|0.670|1.288|1.955|2.224
O.D.: |0.007|0.069|0.161|0.359|0.670|1.288|1.955|2.224
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |83.4|288|1204|95.1|302|1357
Standard Deviation: |3.67|16.13|71.04|5.90|20.54|100.42
CV(%): |4.4|5.6|5.9|6.2|6.8|7.4
n: |16|16|16|24|24|24
Mean(pg/ml): |83.4|288|1204|95.1|302|1357
Standard Deviation: |3.67|16.13|71.04|5.90|20.54|100.42
CV(%): |4.4|5.6|5.9|6.2|6.8|7.4
Assay Procedure Step 1
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human FGF1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human FGF1 standard solution and each sample be measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
(Human FGF1 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human FGF1 PicoKine ELISA Kit standard curve)
Related Product Information for FGF1 elisa kit
For quantitative detection of human FGF1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
Principle: Human FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for FGF1 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: A2-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FGF1 amount of sample captured in plate.
Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
Principle: Human FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for FGF1 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: A2-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FGF1 amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
6,698 Da
NCBI Official Full Name
fibroblast growth factor 1 isoform 1
NCBI Official Synonym Full Names
fibroblast growth factor 1
NCBI Official Symbol
FGF1
NCBI Official Synonym Symbols
AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
NCBI Protein Information
fibroblast growth factor 1
UniProt Protein Name
Fibroblast growth factor 1
UniProt Gene Name
FGF1
UniProt Synonym Gene Names
FGFA; FGF-1; aFGF; ECGF; HBGF-1
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Product Notes
The Human FGF1 fgf1 (Catalog #AAA46757) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46757 ELISA Kit recognizes Human FGF1. It is sometimes possible for the material contained within the vial of "FGF1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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