Standard Curve (Sample)
Human FGF23 ELISA Kit | FGF23 elisa kit
Human FGF23 ELISA Kit
Gene Names
FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
Reactivity
Human
Applications
ELISA
Synonyms
FGF23; N/A; Human FGF23 ELISA Kit; HYPF; Fibroblast growth factor 23; FGF-23; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor; FGF23 elisa kit
Reactivity
Human
Specificity
The Human FGF23 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human FGF23 proteins within the range of 78 pg/ml - 5000 pg/ml.
Sequence Length
251
Samples
Culture Supernatants, Serum, Plasma
Sensitivity
< 30 pg/ml
Applicable Applications for FGF23 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FGF23 elisa kit
Background: FGF23, regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.
Principle of the Assay: The Human FGF23 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF23 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human FGF23 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Human FGF23 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF23 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human FGF23 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,954 Da
NCBI Official Full Name
fibroblast growth factor 23
NCBI Official Synonym Full Names
fibroblast growth factor 23
NCBI Official Symbol
FGF23
NCBI Official Synonym Symbols
ADHR; FGFN; HYPF; HPDR2; PHPTC
NCBI Protein Information
fibroblast growth factor 23; phosphatonin; tumor-derived hypophosphatemia inducing factor
UniProt Protein Name
Fibroblast growth factor 23
UniProt Gene Name
FGF23
UniProt Synonym Gene Names
HYPF; FGF-23
UniProt Entry Name
FGF23_HUMAN
Similar Products
Product Notes
The Human FGF23 fgf23 (Catalog #AAA17870) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17870 ELISA Kit recognizes Human FGF23. AAA Biotech's FGF23 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the FGF23 fgf23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGF23, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is the function of FGF23?
FGF23 is a hormone that regulates phosphate and vitamin D metabolism. It promotes urinary phosphate excretion, suppresses vitamin D activation, and lowers serum phosphate levels. FGF23 works with klotho as a cofactor and plays essential roles in mineral homeostasis and bone health.
What are the diseases associated with FGF23?
Elevated FGF23 is associated with chronic kidney disease, hypertension, cardiovascular disease, bone disease, and X-linked hypophosphatemia. Abnormal FGF23 signaling contributes to mortality risk and progression of renal disease. FGF23 serves as a biomarker for mineral metabolism disorders.
What causes elevated FGF23?
Elevated FGF23 typically results from chronic kidney disease with phosphate retention, vitamin D insufficiency, primary hyperparathyroidism, or genetic disorders like X-linked hypophosphatemia. The kidneys lose responsiveness to FGF23, creating a compensatory feedback loop, causing progressive elevation.
Why does FGF23 inhibit vitamin D?
FGF23 suppresses the 1α-hydroxylase enzyme in kidneys, reducing conversion of inactive vitamin D (25-OHD) to active calcitriol (1,25-OHD2). This mechanism reduces intestinal calcium and phosphate absorption, maintaining phosphate homeostasis and preventing hyperphosphatemia in conditions with phosphate retention.
Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.