Application Data
(This assay recognizes recombinant human FGFR2. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of FGF2, FGF6, FGF7, FGF9, FGF17, FGF21 and LYN at 12ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.)
Human FGFR2/CD332 ELISA Kit | FGFR2/CD332 elisa kit
Human FGFR2/CD332 ELISA Kit
Reactivity
Human
Synonyms
FGFR2/CD332; N/A; Human FGFR2/CD332 ELISA Kit; FGFR2 ELISA Kit, Human; fibroblast growth factor receptor 2; BBDS ELISA Kit, Human; BEK ELISA Kit, Human; BFR-1 ELISA Kit, Human; CD332 ELISA Kit, Human; CEK3 ELISA Kit, Human; CFD1 ELISA Kit, Human; ECT1 ELISA Kit, Human; JWS ELISA Kit, Human; K-SAM ELISA Kit, Human; KGFR ELISA Kit, Human; TK14 ELISA Kit, Human; TK25 ELISA Kit, Human; FGFR2/CD332 elisa kit
Reactivity
Human
Specificity
The kit has been verified by high purity Human FGFR2/CD332 recombinant protein.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Recombinant protein
Detection Range
10.94-700pg/mL
Sensitivity
7.15pg/mL
Conjugation
HRP
Interference
Preparations of FGF2, FGF6, FGF7, FGF9, FGF17, FGF21 and LYN at 12ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes recombinant human FGFR2. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of FGF2, FGF6, FGF7, FGF9, FGF17, FGF21 and LYN at 12ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.)
Application Data
(This assay recognizes recombinant human FGFR2. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of FGF2, FGF6, FGF7, FGF9, FGF17, FGF21 and LYN at 12ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for FGFR2/CD332 elisa kit
This FGFR2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human FGFR2 protein in Recombinant protein. It contains recombinant Human FGFR2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.
Background: FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.
References
Marie PJ, et al. (2003) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. 17(3):877-85.
Park WJ, et al. (1996) Novel FGFR2 mutations in Crouzon and Jackson-Weiss syndromes show allelic heterogeneity and phenotypic variability. Hum Mol Genet. 4(7):1229-33.
Orr-Urtreger A, et al. (1993) Developmental localization of the splicing alternatives of fibroblast growth factor receptor-2 (FGFR2). Dev Biol. 158(2):475-86.
Park WJ, et al. (1996) Novel FGFR2 mutations in Crouzon and Jackson-Weiss syndromes show allelic heterogeneity and phenotypic variability. Hum Mol Genet. 4(7):1229-33.
Orr-Urtreger A, et al. (1993) Developmental localization of the splicing alternatives of fibroblast growth factor receptor-2 (FGFR2). Dev Biol. 158(2):475-86.
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Product Notes
The Human FGFR2/CD332 (Catalog #AAA258392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258392 ELISA Kit recognizes Human FGFR2/CD332. It is sometimes possible for the material contained within the vial of "FGFR2/CD332, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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