Application Data
Human Ficolin-1/FCN1/M Ficolin ELISA Kit | FCN1 elisa kit
Human Ficolin-1/FCN1/M Ficolin Quick ELISA Kit
Gene Names
FCN1; FCNM
Reactivity
Human
Synonyms
Ficolin-1/FCN1/M Ficolin; N/A; Human Ficolin-1/FCN1/M Ficolin Quick ELISA Kit; Ficolin-1|Collagen/fibrinogen domain-containing protein 1; FCN1; Fcna; FCNM; FCNMficolin-A; ficolin (collagen/fibrinogen domain containing) 1; ficolin (collagen/fibrinogen domain-containing) 1; Ficolin1; Ficolin-1; Ficolin-A; ficolin-alpha; M-ficolin; FCN1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human FCN1
Assay Type
Colorimetric sandwich
Samples
cell culture supernatants, serum and plasma (heparin)
Detection Range
0.78ng/ml-50ng/ml
Sensitivity
<20pg/ml
Capture/Detection Antibodies
Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
Sample Data
Concentration(ng/ml)|0|0.78|1.56|3.12|6.25|12.5|25|50
O.D.|0.003|0.177|0.313|0.558|0.926|1.491|1.972|2.357
O.D.|0.003|0.177|0.313|0.558|0.926|1.491|1.972|2.357
Intra-Assay Precision
Sample|1|2|3|
n|16|16|16|
Mean(pg/ml)|1450|8641|19842|
Standard deviation|92.8|475.26|1111.15|
CV(%)|6.4%|5.5%|5.6%|
n|16|16|16|
Mean(pg/ml)|1450|8641|19842|
Standard deviation|92.8|475.26|1111.15|
CV(%)|6.4%|5.5%|5.6%|
Inter-Assay Precision
Sample|1|2|3|
n|24|24|24
Mean(pg/ml)|1321|8694|19651
Standard deviation|93.79|512.95|1061.15
CV(%)|7.1%|5.9%|5.4%
n|24|24|24
Mean(pg/ml)|1321|8694|19651
Standard deviation|93.79|512.95|1061.15
CV(%)|7.1%|5.9%|5.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 1450|1365|1605|1564|1496|97.24|6.5%
Sample 2: 8641|7641|7712|7952|7987|263.55|3.3%
Sample 3: 19842|22647|21642|20458|21147|1141.95|5.4%
*number of samples for each test n=16.
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 1450|1365|1605|1564|1496|97.24|6.5%
Sample 2: 8641|7641|7712|7952|7987|263.55|3.3%
Sample 3: 19842|22647|21642|20458|21147|1141.95|5.4%
*number of samples for each test n=16.
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: A30-A326
Anticoagulant
heparin
Benefits
The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human Ficolin-1/FCN1/M Ficolin with <20pg/ml sensitivity. Format: 96-well plate with removable strips.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
Principle
Human FCN1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FCN1 in cell culture supernatants, serum and plasma (heparin). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human FCN1 with immunogen: Expression system for standard: NS0; Immunogen sequence: A30-A326.
To measure Human FCN1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FCN1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FCN1 in the sample.
To measure Human FCN1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FCN1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FCN1 in the sample.
Assay Procedure Step 1
50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml
TMB Incubation Time
15
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)
Application Data
Application Data
Related Product Information for FCN1 elisa kit
Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34. 3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.
Product Categories/Family for FCN1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
326
NCBI Official Full Name
Ficolin-1
NCBI Official Synonym Full Names
ficolin (collagen/fibrinogen domain containing) 1
NCBI Official Symbol
FCN1
NCBI Official Synonym Symbols
FCNM
NCBI Protein Information
ficolin-1; M-ficolin; ficolin-A; ficolin-alpha; collagen/fibrinogen domain-containing protein 1; ficolin (collagen/fibrinogen domain-containing) 1
UniProt Protein Name
Ficolin-1
UniProt Gene Name
FCN1
UniProt Synonym Gene Names
FCNM
UniProt Entry Name
FCN1_HUMAN
Similar Products
Product Notes
The Human FCN1 fcn1 (Catalog #AAA125893) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125893 ELISA Kit recognizes Human FCN1. It is sometimes possible for the material contained within the vial of "Ficolin-1/FCN1/M Ficolin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.