Human Furin ELISA Kit | FURIN elisa kit

Human Furin PicoKine ELISA Kit

Cat. Num. AAA45544

• Gene Names: FURIN; FUR; PACE; SPC1; PCSK3
• Reactivity: Human
• Synonyms: Furin; N/A; Human Furin PicoKine ELISA Kit; furin (paired basic amino acid cleaving enzyme); Dibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; FUR; FURIN; Furin membrane associated receptor protein; FURIN_HUMAN; PACE; Paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzyme; PCSK3; Proprotein convertase subtilisin/kexin type 3; SPC1; FURIN elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Furin
• Sequence Length: 794
• Samples: Cell culture supernates, tissue homogenates and Cell lysates
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for FURIN elisa kit

Principle of the assay: human Furin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Furin has been precoated onto 96-well plates. Standards(NSO, D108-E715) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Furin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Furin amount of sample captured in plate.
Background: Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

NCBI and Uniprot Product Information

• NCBI GI #: 120611
• NCBI GeneID: 5045
• UniProt Accession #: P09958; Q14336; Q6LBS3; Q9UCZ5
• Molecular Weight: 86,678 Da
• NCBI Official Full Name: Furin
• NCBI Official Synonym Full Names: furin (paired basic amino acid cleaving enzyme)
• NCBI Official Symbol: FURIN
• NCBI Official Synonym Symbols: FUR; PACE; SPC1; PCSK3
• NCBI Protein Information: furin; FES upstream region; dibasic processing enzyme; furin, membrane associated receptor protein; proprotein convertase subtilisin/kexin type 3; paired basic amino acid residue-cleaving enzyme
• UniProt Protein Name: Furin
• UniProt Gene Name: FURIN
• UniProt Synonym Gene Names: FUR; PACE; PCSK3; PACE
• UniProt Entry Name: FURIN_HUMAN