Human G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit | GPER elisa kit

Human G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit

Cat. Num. AAA137454

• Gene Names: GPER1; mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• Reactivity: Human
• Synonyms: G Protein Coupled Estrogen Receptor 1 (GPER); N/A; Human G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit; GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; GPER elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of G Protein Coupled Estrogen Receptor 1 (GPER).; No significant cross-reactivity or interference between G Protein Coupled Estrogen Receptor 1 (GPER) and analogues was observed.
• Sequence Length: 375
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.125ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for GPER elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Estrogen Receptor 1 (GPER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Estrogen Receptor 1 (GPER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GPER elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 148228791
• NCBI GeneID: 2852
• NCBI Accession #: NP_001091671.1
• NCBI GenBank Nucleotide #: NM_001098201.1
• Molecular Weight: 42,248 Da
• NCBI Official Full Name: G-protein coupled estrogen receptor 1
• NCBI Official Synonym Full Names: G protein-coupled estrogen receptor 1
• NCBI Official Symbol: GPER1
• NCBI Official Synonym Symbols: mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• NCBI Protein Information: G-protein coupled estrogen receptor 1
• UniProt Protein Name: G-protein coupled estrogen receptor 1
• UniProt Gene Name: GPER1
• UniProt Synonym Gene Names: CEPR; CMKRL2; DRY12; GPER; GPR30; FEG-1; LYGPR; mER
• UniProt Entry Name: GPER1_HUMAN