Human GDF11 ELISA Kit | GDF11 elisa kit

Human GDF11 ELISA Kit

Cat. Num. AAA263332

• Gene Names: GDF11; BMP11; BMP-11
• Reactivity: Human
• Synonyms: GDF11; N/A; Human GDF11 ELISA Kit; BMP11; Growth/differentiation factor 11; GDF-11; Bone morphogenetic protein 11; BMP-11; GDF11 elisa kit

• Reactivity: Human
• Specificity: The Human GDF11 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human GDF11 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 407
• Samples: Culture Supernates, Serum, Plasma
• Sensitivity: < 15 pg/ml

• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for GDF11 elisa kit

Background: Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid prepropeptide which contains a signal sequence for secretion and an RXXR proteolytic processing site to yield a 109 aa residue carboxy-terminal mature protein. Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. As detected by in situ hybridization, GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain. Likewise, a targeted deletion of GDF-11 in mice results in a spectrum of abnormalities including palatal malformation, vertebral defects, elongated trunks with a reduced or absent tail, missing or malformed kidneys, and an increased number of neurons in the olfactory epithelium. An intriguing finding in the knockout mice was that the trunk elongation was due to an increase in the number of thoracic vertebrae. This implicates GDF-11 as the first secreted factor to influence the specification of segmental identity in vertebrates. In fact, GDF-11 does regulate expression of segmental transcription factors, the Hox genes. GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning. Despite the strong expression in the limb throughout development, no limb abnormalities were found in the knockout mice. However, in vitro micromass studies indicate that GDF-11 inhibits myogenic and chondrogenic cell differentiation and may impact formation and development of the limb skeleton.

Principle of the Assay: The AAA Biotech' Human GDF11 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GDF11 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human GDF11 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDF11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 5031613
• NCBI GeneID: 10220
• NCBI Accession #: NP_005802.1
• NCBI GenBank Nucleotide #: NM_005811.4
• UniProt Accession #: O95390; Q9UID1; Q9UID2
• Molecular Weight: 45,091 Da
• NCBI Official Full Name: growth/differentiation factor 11 preproprotein
• NCBI Official Synonym Full Names: growth differentiation factor 11
• NCBI Official Symbol: GDF11
• NCBI Official Synonym Symbols: BMP11; BMP-11
• NCBI Protein Information: growth/differentiation factor 11
• UniProt Protein Name: Growth/differentiation factor 11
• UniProt Gene Name: GDF11
• UniProt Synonym Gene Names: BMP-11