Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit | GRIN1 elisa kit

Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit

Cat. Num. AAA186692

• Gene Names: GRIN1; NR1; MRD8; GluN1; NMDA1; NMD-R1; NMDAR1
• Reactivity: Human
• Synonyms: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1); N/A; Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit; NMDA1; NMDAR1; NR1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; GRIN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1).; No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) and analogues was observed.
• Sequence Length: 938
• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for GRIN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11038637
• NCBI GeneID: 2902
• NCBI Accession #: NP_015566.1
• NCBI GenBank Nucleotide #: NM_007327.3
• UniProt Accession #: Q05586; P35437; Q12867; Q12868; Q5VSF3; Q5VSF4; Q5VSF5; Q5VSF6; Q5VSF7; A6NLK7; A6NLR1; C9K0X1
• Molecular Weight: 101,851 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 1 isoform GluN1-1a
• NCBI Official Synonym Full Names: glutamate ionotropic receptor NMDA type subunit 1
• NCBI Official Symbol: GRIN1
• NCBI Official Synonym Symbols: NR1; MRD8; GluN1; NMDA1; NMD-R1; NMDAR1
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 1
• UniProt Gene Name: GRIN1
• UniProt Synonym Gene Names: NMDAR1; GluN1; NMD-R1