Human GM-CSF ELISA Kit | GM-CSF elisa kit

Human GM-CSF FAST ELISA Kit

Cat. Num. AAA282452

• Reactivity: Human
• Synonyms: GM-CSF; N/A; Human GM-CSF FAST ELISA Kit; GM-CSF elisa kit

• Reactivity: Human
• Specificity: This assay recognizes both recombinant and natural human GM-CSF. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8-500pg/mL
• Sensitivity: < 6.4pg/mL

• Intra-assay Precision: Intra-plate Precision 3 samples with low, middle and high level GM-CSF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-plate Precision 3 samples with low, middle and high level GM-CSF were tested on 3 different plates, 20 replicates in each plate. Inter-Assay: CV<15%
• Preparation and Storage: Ship: Ice Pack

Standard Curve (Sample)

Related Product Information for GM-CSF elisa kit

Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GM-CSF in human serum, plasma, cell culture supernatants and other biological fluids.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human GM-CSF has been pre-coated onto a microplate.Samples (or Standards) and biotinylated detection antibody specific for GM-CSF are added to the wells, GM-CSF binds to the antibody on the solid-phase vector and the detection antibody to complete the "one-step sandwich". After incubation, the wells are washed to remove unbound material, and Avidin-Horseradish Peroxidase (HRP) conjugate is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the absorbance is measured.