Human Group IIE secretory phospholipase A2 (PLA2G2E) ELISA Kit | PLA2G2E elisa kit
Human Group IIE secretory phospholipase A2 (PLA2G2E) ELISA Kit
Gene Names
PLA2G2E; sPLA2-IIE; GIIE sPLA2
Reactivity
Human
Synonyms
Group IIE secretory phospholipase A2 (PLA2G2E); N/A; Human Group IIE secretory phospholipase A2 (PLA2G2E) ELISA Kit; PLA2G2E elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PLA2G2E. No significant cross-reactivity or interference between Human PLA2G2E and analogues was observed.
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.078ng/mL
Detection Wavelength
450nm
Assay Duration
1-4.5h
Assay Duration
1-4.5h
Sample Volume
1-200uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate PLA2G2E in samples. An antibody specific for PLA2G2E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G2E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G2E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G2E bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,989 Da
NCBI Official Full Name
group IIE secretory phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group IIE
NCBI Official Symbol
PLA2G2E
NCBI Official Synonym Symbols
sPLA2-IIE; GIIE sPLA2
NCBI Protein Information
group IIE secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 2E
UniProt Protein Name
Group IIE secretory phospholipase A2
UniProt Gene Name
PLA2G2E
UniProt Synonym Gene Names
GIIE sPLA2; sPLA2-IIE
UniProt Entry Name
PA2GE_HUMAN
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Product Notes
The Human PLA2G2E pla2g2e (Catalog #AAA190409) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA190409 ELISA Kit recognizes Human PLA2G2E. It is sometimes possible for the material contained within the vial of "Group IIE secretory phospholipase A2 (PLA2G2E), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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