Human GSTpi ELISA Kit | GSTpi elisa kit
Human GSTpi (Glutathione S Transferases Pi) ELISA Kit
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
Human
Synonyms
GSTpi; N/A; Human GSTpi (Glutathione S Transferases Pi) ELISA Kit; GSTpi elisa kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinantHumanGSTpi. No significant cross-reactivity or interference between HumanGSTpi and analogues was observed.
Sequence Length
210
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
2.344-150ng/mL
Sensitivity
Min: 1.406ng/mL; Max: 150ng/mL
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for GSTpi elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanGSTpiconcentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GSTpi. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GSTpiand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GSTpi, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GSTpi.You can calculate the concentration of GSTpiin the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GSTpi. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GSTpiand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GSTpi, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GSTpi.You can calculate the concentration of GSTpiin the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,356 Da
NCBI Official Full Name
glutathione S-transferase-P1c
NCBI Official Synonym Full Names
glutathione S-transferase pi 1
NCBI Official Symbol
GSTP1
NCBI Official Synonym Symbols
PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
NCBI Protein Information
glutathione S-transferase P; GST class-pi; GSTP1-1; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III
UniProt Protein Name
Glutathione S-transferase P
UniProt Gene Name
GSTP1
UniProt Synonym Gene Names
FAEES3; GST3
UniProt Entry Name
GSTP1_HUMAN