Human GTP-binding protein Rheb (RHEB) ELISA Kit | RHEB elisa kit
Human GTP-binding protein Rheb (RHEB) ELISA Kit
Gene Names
RHEB; RHEB2
Reactivity
Human
Synonyms
GTP-binding protein Rheb (RHEB); N/A; Human GTP-binding protein Rheb (RHEB) ELISA Kit; MGC111559; RHEB2; GTP-binding protein Rheb|Ras homolog enriched in brain 2; RHEB elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human RHEB. No significant cross-reactivity or interference between Human RHEB and analogues was observed.
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other biological fluids
Detection Range
0.625-40ng/mL
Sensitivity
0.31ng/mL
Detection Wavelength
450nm
Assay Duration
1-4.5h
Assay Duration
1-4.5h
Sample Volume
1-200uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate RHEB in samples. An antibody specific for RHEB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RHEB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHEB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHEB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
Related Product Information for RHEB elisa kit
Rheb is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins.
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
184
NCBI Official Full Name
GTP-binding protein Rheb
NCBI Official Synonym Full Names
Ras homolog enriched in brain
NCBI Official Symbol
RHEB
NCBI Official Synonym Symbols
RHEB2
NCBI Protein Information
GTP-binding protein Rheb; Ras homolog enriched in brain 2
UniProt Protein Name
GTP-binding protein Rheb
UniProt Gene Name
RHEB
UniProt Synonym Gene Names
RHEB2
UniProt Entry Name
RHEB_HUMAN
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Product Notes
The Human RHEB rheb (Catalog #AAA190404) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA190404 ELISA Kit recognizes Human RHEB. It is sometimes possible for the material contained within the vial of "GTP-binding protein Rheb (RHEB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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