Human Heart-type Fatty Acid Binding Protein (H-FABP) ELISA Kit | H-FABP elisa kit

Human Heart-type Fatty Acid Binding Protein (H-FABP) High Sensitive ELISA Kit

Cat. Num. AAA188540

• Gene Names: FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
• Reactivity: Human
• Applications: ELISA
• Synonyms: Heart-type Fatty Acid Binding Protein (H-FABP); N/A; Human Heart-type Fatty Acid Binding Protein (H-FABP) High Sensitive ELISA Kit; Heart-type Fatty Acid Binding Protein; MDGI; FABP11; H-FABP; O-FABP; Hfabp; FABP3; Fatty Acid Binding Protein 3, Muscle And Heart; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-ty; H-FABP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP).; No significant cross-reactivity or interference between High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) and analogues was observed.
• Sequence Length: 133
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 24.7pg/mL

• Applicable Applications for H-FABP elisa kit: ELISA
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 119802
• NCBI GeneID: 2170
• UniProt Accession #: P05413
• NCBI Official Full Name: Fatty acid-binding protein, heart
• NCBI Official Synonym Full Names: fatty acid binding protein 3
• NCBI Official Symbol: FABP3
• NCBI Official Synonym Symbols: MDGI; FABP11; H-FABP; M-FABP; O-FABP
• NCBI Protein Information: fatty acid-binding protein, heart
• UniProt Protein Name: Fatty acid-binding protein, heart
• UniProt Gene Name: FABP3
• UniProt Synonym Gene Names: FABP11; MDGI; H-FABP; MDGI; M-FABP
• UniProt Entry Name: FABPH_HUMAN