Standard Curve (Sample)
(Fig.1. Human Hepatocyte growth factor receptor (c-MET/HGFR) Standard Curve.)
Human Hepatocyte Growth Factor Receptor (c-MET/HGFR) ELISA Kit | HGFR elisa kit
Human Hepatocyte Growth Factor Receptor (c-MET/HGFR) ELISA Kit
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human
Synonyms
Hepatocyte Growth Factor Receptor (c-MET/HGFR); N/A; Human Hepatocyte Growth Factor Receptor (c-MET/HGFR) ELISA Kit; MET; AUTS9; RCCP2; c-Met; HGF receptor; SF receptor; met proto-oncogene; met proto-oncogene tyrosine kinase; oncogene MET; scatter factor receptor; HGFR elisa kit
Reactivity
Human
Specificity
Human Hepatocyte growth factor receptor (c-MET/HGFR) ELISA Kit has high sensitivity and excellent specificity for detection of Human MET. No significant cross-reactivity or interference between Human MET and analogues was observed.
Sequence Length
1390
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Hepatocyte growth factor receptor (c-MET/HGFR) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Hepatocyte growth factor receptor (c-MET/HGFR) Standard Curve.)
Related Product Information for HGFR elisa kit
Description: This Human Hepatocyte growth factor receptor (c-MET/HGFR) ELISA Kit employs a two-site sandwich ELISA to quantitate MET in samples. An antibody specific for MET has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MET present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MET is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MET bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MET encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of METare also associated with multiple human cancers.
Background: MET encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of METare also associated with multiple human cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
hepatocyte growth factor receptor isoform b preproprotein
NCBI Official Synonym Full Names
MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol
MET
NCBI Official Synonym Symbols
HGFR; AUTS9; RCCP2; c-Met; DFNB97
NCBI Protein Information
hepatocyte growth factor receptor
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Gene Name
MET
UniProt Synonym Gene Names
HGF receptor; SF receptor
UniProt Entry Name
MET_HUMAN
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Product Notes
The Human HGFR met (Catalog #AAA331794) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331794 ELISA Kit recognizes Human HGFR. It is sometimes possible for the material contained within the vial of "Hepatocyte Growth Factor Receptor (c-MET/HGFR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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