Standard Curve (Sample)
Human HGF ELISA Kit | HGF elisa kit
Human HGF ELISA Kit
Gene Names
HGF; SF; HGFB; HPTA; F-TCF; DFNB39
Reactivity
Human
Applications
ELISA
Synonyms
HGF; N/A; Human HGF ELISA Kit; DFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatopoietin A; Hepatopoietin-A; Hgf; HGFB; HPTA; HGF elisa kit
Reactivity
Human
Specificity
Human HGF ELISA Kit has high sensitivity and excellent specificity for detection of Human HGF. No significant cross-reactivity or interference between Human HGF and analogues was observed.
Assay Type
Sandwich
Samples
Serum, Plasma, Cell culture supernatants
Detection Range
125 pg/mL-8,000 pg/mL
Sensitivity
60 pg/mL
Applicable Applications for HGF elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision: The CV (%)?10%.
Inter-assay Precision
Inter-assay Precision :The CV (%)?12%
Preparation and Storage
The unopened kit should be stored at 2 - 8 degree C. After opening, please store refer to protocols.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HGF elisa kit
Principle of the Assay: HGF gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin.Human HGF ELISA Kit employs a double antibody sandwich method to quantitate Human HGF in samples. An antibody specific for Human HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Human HGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450nm wavelength. The HGF concentration is proportional to the OD450 nm value.
Product Categories/Family for HGF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
728
NCBI Official Full Name
Hepatocyte growth factor
NCBI Official Synonym Full Names
hepatocyte growth factor (hepapoietin A; scatter factor)
NCBI Official Symbol
HGF
NCBI Official Synonym Symbols
SF; HGFB; HPTA; F-TCF; DFNB39
NCBI Protein Information
hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
UniProt Protein Name
Hepatocyte growth factor
UniProt Gene Name
HGF
UniProt Synonym Gene Names
HPTA; SF
UniProt Entry Name
HGF_HUMAN
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Product Notes
The Human HGF hgf (Catalog #AAA332538) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332538 ELISA Kit recognizes Human HGF. AAA Biotech's HGF can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the HGF hgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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