Human IDS ELISA Kit | IDS elisa kit

Human IDS ELISA Kit

Cat. Num. AAA263051

• Gene Names: IDS; MPS2; SIDS
• Reactivity: Human
• Synonyms: IDS; N/A; Human IDS ELISA Kit; SIDS; Iduronate 2-sulfatase; Alpha-L-iduronate sulfate sulfatase; Idursulfase; IDS elisa kit

• Reactivity: Human
• Specificity: The Human IDS ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IDS proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 550
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml

• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for IDS elisa kit

Principle of the Assay: The Human IDS ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IDS in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human IDS coated on a 96-well plate. Standards and samples are pipetted into the wells and IDS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IDS antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IDS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

NCBI and Uniprot Product Information

• NCBI GI #: 4557659
• NCBI GeneID: 3423
• NCBI Accession #: NP_000193.1
• NCBI GenBank Nucleotide #: NM_000202.7
• UniProt Accession #: P22304; Q14604; Q9BRM3; D3DWT4
• Molecular Weight: 34,893 Da
• NCBI Official Full Name: iduronate 2-sulfatase isoform a preproprotein
• NCBI Official Synonym Full Names: iduronate 2-sulfatase
• NCBI Official Symbol: IDS
• NCBI Official Synonym Symbols: MPS2; SIDS
• NCBI Protein Information: iduronate 2-sulfatase
• UniProt Protein Name: Iduronate 2-sulfatase
• UniProt Gene Name: IDS
• UniProt Synonym Gene Names: SIDS; Idursulfase
• UniProt Entry Name: IDS_HUMAN