Human IGFBP-3 ELISA Kit | IGFBP-3 elisa kit

IGFBP-3 Human ELISA Kit

Cat. Num. AAA59040

• Gene Names: IGFBP3; IBP3; BP-53
• Reactivity: Human
• Applications: ELISA
• Synonyms: IGFBP-3; N/A; IGFBP-3 Human ELISA Kit; Human IGFBP-3; IGFBP-3 elisa kit

• Reactivity: Human
• Assay Type: Sandwich
• Samples: Human serum, plasma, body fluids, tissue lysates or cell culture supernatants
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 10 pg/ml

• Applicable Applications for IGFBP-3 elisa kit: ELISA
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Standard Curve (Sample)

Related Product Information for IGFBP-3 elisa kit

Background: Insulin-like growth factor-binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor-binding protein (IGFBP) family. This protein has an IGFBP domain and a thyroglobulin type-I domain. It forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. It plays a role in controlling angiogenesis and cell responses in the human corpus luteum by autocrine/paracrine mechanisms. IGFBP3 protein levels decrease during the progression of prostate cancer from benign prostatic hyperplasia (BPH) to its metastatic form.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IGFBP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGFBP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP-3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 62243248
• NCBI GeneID: 3486
• NCBI Accession #: NP_001013416.1
• NCBI GenBank Nucleotide #: NM_001013398.1
• UniProt Accession #: P17936; Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0
• Molecular Weight: 31,674 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 3 isoform a
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IBP3; BP-53
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_HUMAN