Human IGFBP7 ELISA Kit | IGFBP7 elisa kit

Human IGFBP7 PicoKine ELISA Kit

Cat. Num. AAA11629

• Gene Names: IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• Reactivity: Human
• Synonyms: IGFBP7; N/A; Human IGFBP7 PicoKine ELISA Kit; Insulin-like growth factor-binding protein 7; insulin-like growth factor binding protein 7; AGM; Angiomodulin; FSTL2; IBP-7; IBP7; IBP7_HUMAN; IGF binding protein 7; IGF-binding protein 7; IGFBP rP1; IGFBP-7; IGFBP-rP1; IGFBPRP1; Insulin like growth factor binding protein 7; MAC25; MAC25 protein; PGI2 stimulating factor; PGI2-stimulating factor; Prostacyclin stimulating factor; Prostacyclin-stimulating factor; PSF; RAMSVPS; TAF; Tumor-derived adhesion factor; IGFBP7 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IGFBP7
• Sequence Length: 282
• Samples: Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
• Detection Range: 625 pg/ml - 40,000 pg/ml
• Sensitivity: <20 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for IGFBP7 elisa kit

Background: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Principle of the Assay: The Picokine™ Human IGFBP7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IGFBP7 with a 96-well strip plate that is pre-coated with antibody specific for IGFBP7. The detection antibody is a biotinylated antibody specific for IGFBP7. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit includes Human IGFBP7 protein as standards. To measure Human IGFBP7, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IGFBP7 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IGFBP7 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 23396609
• NCBI GeneID: 3490
• UniProt Accession #: Q16270; Q07822; Q53YE6; Q9UCA8; B4E1N2; B7Z9W7
• Molecular Weight: 28,860 Da
• NCBI Official Full Name: Insulin-like growth factor-binding protein 7
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 7
• NCBI Official Symbol: IGFBP7
• NCBI Official Synonym Symbols: AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• NCBI Protein Information: insulin-like growth factor-binding protein 7; IGFBP-rP1; angiomodulin; IGF-binding protein 7; PGI2-stimulating factor; tumor-derived adhesion factor; prostacyclin-stimulating factor
• UniProt Protein Name: Insulin-like growth factor-binding protein 7
• UniProt Gene Name: IGFBP7
• UniProt Synonym Gene Names: MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF
• UniProt Entry Name: IBP7_HUMAN