Standard Curve (Sample)
Human IgG total ELISA Kit | IgG elisa kit
Human IgG total ELISA Kit
Reactivity
Human
Synonyms
IgG total; N/A; Human IgG total ELISA Kit; IgG elisa kit
Reactivity
Human
Specificity
This assay recognizes both natural and recombinant human IgG total.
Assay Type
Quantitative Sandwich
Samples
Cell culture supernates, serum, and plasma
Sensitivity
0.39ng/mL
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IgG elisa kit
Background: Immunoglobulin G (IgG) is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG has two antigen binding sites. IgG are involved in the regulation of allergic reactions. According to Finkelman, there are two pathways of systemic anaphylaxis: antigens can cause systemic anaphylaxis in mice through classic pathway by cross-linking IgE bound to the mast cell receptor FcepsilonRI, stimulating the release of both histamine and platelet activating factor (PAF). In the alternative pathway antigens form complexes with IgG, which then cross-link macrophage receptor FcgammaRIII and stimulates only PAF release. Human IgG is the major immunoglobulin in blood and plays a key role in the humoral immune response.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IgG total has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG total present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then the HRP-Conjugate anti-human IgG total monoclonal antibody is added to the wells and binds to the combination of capture antibodyIgG total in sample. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IgG total present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IgG total standard dilutions and IgG total sample concentration determined.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IgG total has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG total present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then the HRP-Conjugate anti-human IgG total monoclonal antibody is added to the wells and binds to the combination of capture antibodyIgG total in sample. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IgG total present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IgG total standard dilutions and IgG total sample concentration determined.
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Product Notes
The Human IgG (Catalog #AAA313153) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA313153 ELISA Kit recognizes Human IgG. It is sometimes possible for the material contained within the vial of "IgG total, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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