Standard Curve (Sample)
Human IgG4 ELISA Kit | IgG4 elisa kit
Human IgG4 ELISA Kit
Reactivity
Human
Synonyms
IgG4; N/A; Human IgG4 ELISA Kit; IgG4 elisa kit
Reactivity
Human
Specificity
This assay recognizes both recombinant and natural human IgG4. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity. No significant cross-reactivity was observed with the following. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between IgG4 and all the analogues, therefore, cross reaction may still exist.
Assay Type
Quantitative Sandwich
Sensitivity
< 7.8ng/mL
Intra-assay Precision
Intra-plate Precision 3 samples with low, middle and high level IgG4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-plate Precision 3 samples with low, middle and high level IgG4 were tested on 3 different plates, 20 replicates in each plate. Inter-Assay: CV<15%
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IgG4 elisa kit
Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IgG4 in human serum, plasma, cell culture supernatants, cell lysates, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for IgG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG4 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and enzyme conjugate detection antibody is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of IgG4 bound in the initial step. The color development is stopped and the absorbance is measured.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for IgG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG4 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and enzyme conjugate detection antibody is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of IgG4 bound in the initial step. The color development is stopped and the absorbance is measured.
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Product Notes
The Human IgG4 (Catalog #AAA281403) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281403 ELISA Kit recognizes Human IgG4. It is sometimes possible for the material contained within the vial of "IgG4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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