Human IL-12 p35 ELISA Kit | IL-12 p35 elisa kit

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit

Cat. Num. AAA21922

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Reactivity: Human
• Synonyms: IL-12 p35; N/A; Human IL-12 p35 (Interleukin 12 p35) ELISA Kit; IL-12 p35 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human IL-12 p35 in samples. No significant cross-reactivity or interference between Human IL-12 p35 and analogues was observed.
• Sequence Length: 253
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 7.81-500pg/mL
• Sensitivity: 4.69pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-12 p35 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-12 p35 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Standard Curve (Sample)

Related Product Information for IL-12 p35 elisa kit

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12 p35. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 p35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12 p35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-12 p35. You can calculate the concentration of Human IL-12 p35 in the samples by comparing the OD of the samples to the standard curve.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-12 p35 concentrations in serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 24430219
• NCBI GeneID: 3592
• NCBI Accession #: NP_000873.2
• NCBI GenBank Nucleotide #: NM_000882.3
• Molecular Weight: 24,874 Da
• NCBI Official Full Name: interleukin-12 subunit alpha
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha; CLMF p35; IL-12, subunit p35; IL35 subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1
• UniProt Entry Name: IL12A_HUMAN