Human IL-12(P70) ELISA Kit | IL-12(P70) elisa kit

Human IL-12(P70) PicoKine Quick ELISA Kit

Cat. Num. AAA128054

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-12(P70); N/A; Human IL-12(P70) PicoKine Quick ELISA Kit; IL-12(P70) elisa kit

• Reactivity: Human
• Samples: cell culture supernatants and serum
• Assay Range: 7.8pg/ml-500pg/ml
• Sensitivity: <2pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for IL-12(P70) elisa kit: ELISA
• Kit Components: Description/Quantity/Volume; Pre-coated 96-well Strip Microplate/1/12 strips of 8 wells; Standard/2, 10 ng/tube; HRP-Linked Antibody/1/6ml; Sample Diluent/1/15ml; TBS-T Wash Buffer (25x)/1/12ml; Color Developing Reagent (TMB)/1/10ml; Stop Solution/1/10ml; Adhesive plate sealers/2/Piece
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Automated plate washer (optional); Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.; Horizontal orbital microplate shaker capable of maintaining a speed of 500rpm, amplitude 3mm.
• Sample Data:

  Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
  O.D.	0.021	0.088	0.140	0.274	0.513	0.953	1.620	2.044

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	18	51	294	19	55	310
  Standard deviation	1.31	3.41	17.38	1.67	3.79	21.39
  CV (%)	7.3%	6.7%	5.2%	8.8%	6.9%	6.9%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	18	18	20	17	18	1.08	6%
  Sample 2	51	59	60	54	56	3.67	6.5%
  Sample 3	294	290	284	296	291	4.58	1.5%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human IL-12(P70) PicoKine Quick ELISA Kit standard curve)

Related Product Information for IL-12(P70) elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for IL-12(P70) elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 24430219
• NCBI GeneID: 3592
• NCBI Accession #: NP_000873.2
• NCBI GenBank Nucleotide #: NM_000882.3
• UniProt Accession #: P29459; Q96QZ1
• Molecular Weight: 24,874 Da
• NCBI Official Full Name: interleukin-12 subunit alpha
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lym
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1
• UniProt Entry Name: IL12A_HUMAN