Human IL-2 ELISA Kit | IL-2 elisa kit

Human IL-2 PicoKine Quick ELISA Kit

Cat. Num. AAA128052

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-2; N/A; Human IL-2 PicoKine Quick ELISA Kit; Interleukin-2; T-cell growth factor; TCGF; Aldesleukin; IL2; IL-2 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma ( heparin, EDTA, citrate)
• Assay Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <1pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: A21-T153
• Anticoagulant: heparin, EDTA or citrate
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for IL-2 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.030	0.065	0.123	0.212	0.336	0.609	1.031	1.798

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	31	158	484	28	169	474
  Standard deviation	1.27	8.84	30	1.14	10.98	38.39
  CV (%)	4.1%	5.6%	6.2%	4.1%	6.5%	8.1%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	31	29	28	30	29	1.11	3.8%
  Sample 2	158	160	151	163	158	4.41	2.7%
  Sample 3	484	469	488	480	480	7.08	1.4%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human IL-2 PicoKine ELISA Kit standard curve)

Related Product Information for IL-2 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for IL-2 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 28178861
• NCBI GeneID: 3558
• NCBI Accession #: NP_000577.2
• NCBI GenBank Nucleotide #: NM_000586.3
• UniProt Accession #: P60568; P01585
• Molecular Weight: 17,628 Da
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2; aldesleukin; T cell growth factor; involved in regulation of T-cell clonal expansion
• UniProt Protein Name: Interleukin-2
• UniProt Gene Name: IL2
• UniProt Synonym Gene Names: IL-2; TCGF
• UniProt Entry Name: IL2_HUMAN