Human IL-20 ELISA Kit | IL-20 elisa kit

Human IL-20 ELISA Kit

Cat. Num. AAA258453

• Reactivity: Human
• Synonyms: IL-20; N/A; Human IL-20 ELISA Kit; IL-20 ELISA Kit, Human; interleukin 20; IL10D ELISA Kit, Human; interleukin-20 ELISA Kit, Human; ZCYTO10 ELISA Kit, Human; IL-20 elisa kit

• Reactivity: Human
• Specificity: For the quantitative determination of Human IL-20 concentration in cell culture supernates.
• Assay Type: Solid Phase Sandwich ELISA (quantitative)
• Sample Types: Cell supernatant
• Detection Range: 15.63-1000pg/mL
• Sensitivity: 7.99pg/mL
• Conjugation: HRP
• Interference: Preparations of the factors listed below at 50ng/mL in a mid-range recombinant human IL20 control were assayed for interference. No significant interference was observed.
• Materials Provided: 1. 96 well microplate coated with Capture Antibody; 2. Detection Antibody conjugated to HRP; 3. Standards; 4. Wash Buffer Concentrate; 5. Dilution Buffer Concentrate; 6. Color Reagent A; 7. Color Reagent B; 8. Stop Solution

Application Data

(This assay recognizes both recombinant and natural Human IL20. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample)

(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

Related Product Information for IL-20 elisa kit

This IL-20 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-20 protein in Cell supernatant. It contains recombinant Human IL-20, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: IL20/Interleukin-20 belongs to the IL-10 family. It is a cytokine structurally related to interleukin 10. IL20/Interleukin-20 can be detected in skin, trachea, and other tissues. It is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. It has been shown that interleukin-20 transduces its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. It may be involved in epidermal function and psoriasis. It also regulates the proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Also, IL20/Interleukin-20 also causes cell expansion of multipotential hematopoietic progenitor cells. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

References

Chen XY, et al. (2011) The association between the IL-20-1723CG allele on the 1q chromosome and psoriasis triggered or exacerbated by an upper respiratory tract infection in the Chinese Han population. Dermatology. 222(1):24-30.
Baird AM, et al. (2011) IL-20 is epigenetically regulated in NSCLC and down regulates the expression of VEGF. Eur J Cancer. 47(12):1908-18.
Logsdon NJ, et al. (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the IL-20-IL-20R1-IL-20R2 complex. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 1):89-92.