Standard Curve (Sample)
Human Insulin ELISA Kit | INS elisa kit
Insulin: Human (ELISA)
Gene Names
INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
Reactivity
Human
Synonyms
Insulin; N/A; Insulin: Human (ELISA); Human Insulin; INS elisa kit
Reactivity
Human
Sequence Length
110
Samples
Human serum and plasma samples
Sensitivity
3uU/ml
Intra-assay Precision
C.V. < 10%.
Inter-assay Precision
C.V. <10%.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for INS elisa kit
Principle of the Assay: This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and coincubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
References
1. Molecular mechanisms of insulin resistance in diabetes. Soumaya K. Adv Exp Med Biol. 2012;771:240-51.
2. The links between insulin resistance, diabetes, and cancer. Orgel E, Mittelman SD. Curr Diab Rep. 2013 13(2):213-22
2. The links between insulin resistance, diabetes, and cancer. Orgel E, Mittelman SD. Curr Diab Rep. 2013 13(2):213-22
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Human INS (Catalog #AAA60546) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA60546 ELISA Kit recognizes Human INS. It is sometimes possible for the material contained within the vial of "Insulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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