Human Interferon Kappa (IFNk) ELISA Kit | IFNk elisa kit

Human Interferon Kappa (IFNk)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA23128

• Gene Names: IFNK; IFNT1; INFE1
• Reactivity: Human
• Applications: ELISA
• Synonyms: Interferon Kappa (IFNk); N/A; Human Interferon Kappa (IFNk)(Sandwich ELISA) ELISA Kit; ELISA Kit for Interferon Kappa (IFNk); IFNk/Interferon Kappa; IFN-K; IFNk elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IFNk. No significant cross-reactivity or interference between IFNk and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: < 3.1pg/mL

• Applicable Applications for IFNk elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFNk were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFNk were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for IFNk elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IFNk. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IFNk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFNk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IFNk in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFNk in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for IFNk elisa kit

Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 112789564
• NCBI GeneID: 56832
• NCBI Accession #: NP_064509.2
• NCBI GenBank Nucleotide #: NM_020124.2
• UniProt Accession #: Q9P0W0; Q5T166
• Molecular Weight: 25,218 Da
• NCBI Official Full Name: interferon kappa
• NCBI Official Synonym Full Names: interferon kappa
• NCBI Official Symbol: IFNK
• NCBI Official Synonym Symbols: IFNT1; INFE1
• NCBI Protein Information: interferon kappa
• UniProt Protein Name: Interferon kappa
• UniProt Gene Name: IFNK
• UniProt Synonym Gene Names: IFN-kappa