Standard Curve (Sample)
Human JAM-C ELISA Kit | JAM-C elisa kit
Human JAM-C PicoKine ELISA Kit
Gene Names
JAM3; JAMC; JAM-2; JAM-3; JAM-C
Reactivity
Human
Synonyms
JAM-C; N/A; Human JAM-C PicoKine ELISA Kit; Human JAM-C; JAM-C elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human JAM2
Sequence Length
259
Samples
Cell Culture Supernates, Serum And Plasma(Heparin, Edta)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for JAM-C elisa kit
Principle of the Assay: The Picokine Human JAM2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human JAM2 with a 96-well strip plate that is pre-coated with antibody specific for JAM2. The detection antibody is a biotinylated antibody specific for JAM2. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human JAM2 with immunogen: Expression system for standard: NSO, Immunogen sequence: F29-S238. The kit is analytically validated with ready to use reagents.To measure Human JAM2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human JAM2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human JAM2 in the sample!!Background/Introduction: Junctional adhesion molecule B is a protein that in humans is encoded by the JAM2 gene. It is mapped to 21q21.3. This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
junctional adhesion molecule C isoform 2
NCBI Official Synonym Full Names
junctional adhesion molecule 3
NCBI Official Symbol
JAM3
NCBI Official Synonym Symbols
JAMC; JAM-2; JAM-3; JAM-C
NCBI Protein Information
junctional adhesion molecule C
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Product Notes
The Human JAM-C (Catalog #AAA125072) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125072 ELISA Kit recognizes Human JAM-C. It is sometimes possible for the material contained within the vial of "JAM-C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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