Standard Curve (Sample)
(Fig.1. Human Kallikrein 8 (KLK8) Standard Curve.)
Human Kallikrein 8 (KLK8) ELISA Kit | KLK8 elisa kit
Human Kallikrein 8 (KLK8) ELISA Kit
Gene Names
KLK8; NP; HNP; NRPN; PRSS19; TADG14
Reactivity
Human
Synonyms
Kallikrein 8 (KLK8); N/A; Human Kallikrein 8 (KLK8) ELISA Kit; KLK8; HNP; NP; NRPN; PRSS19; TADG14; KLK8 protein type 1; KLK8 protein type 2; kallikrein 8; neuropsin type 1; neuropsin type 2; ovasin; serine protease 19; tumor-associated differentially expressed gene 14; KLK8 elisa kit
Reactivity
Human
Specificity
Human Kallikrein 8 (KLK8) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK8. No significant cross-reactivity or interference between Human KLK8 and analogues was observed.
Sequence Length
260
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Kallikrein 8 (KLK8) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Kallikrein 8 (KLK8) Standard Curve.)
Related Product Information for KLK8 elisa kit
Description: This Human Kallikrein 8 (KLK8) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK8 in samples. An antibody specific for KLK8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLK8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Originally described from mouse brain, KLK8 was implicated in neural plasticity and learning. Kallikrein 8 is thought to be involved in neurite outgrowth and fasciculation during nervous system development. Kallikrein 8 is induced in neurite injury, and Kallikrein 8 has been shown to be elevated in the hippocampus on Alzheimer's patients. Kallikrein 8 is thought to regulate the early phase of long-term potentiation (LTP), possibly by cleaving fibronectin and other substrates.Kallikrein 8 is also found in the uterus, where it is hormonally regulated. Cervical cancer cell lines express high levels of Kallikrein 8, and it may be useful as a tumor marker. Kallikrein 8 is not limited to the brain and uterus, as many tissues and cell lines make the protein.
Background: Originally described from mouse brain, KLK8 was implicated in neural plasticity and learning. Kallikrein 8 is thought to be involved in neurite outgrowth and fasciculation during nervous system development. Kallikrein 8 is induced in neurite injury, and Kallikrein 8 has been shown to be elevated in the hippocampus on Alzheimer's patients. Kallikrein 8 is thought to regulate the early phase of long-term potentiation (LTP), possibly by cleaving fibronectin and other substrates.Kallikrein 8 is also found in the uterus, where it is hormonally regulated. Cervical cancer cell lines express high levels of Kallikrein 8, and it may be useful as a tumor marker. Kallikrein 8 is not limited to the brain and uterus, as many tissues and cell lines make the protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kallikrein-8 isoform 1 preproprotein
NCBI Official Synonym Full Names
kallikrein related peptidase 8
NCBI Official Symbol
KLK8
NCBI Official Synonym Symbols
NP; HNP; NRPN; PRSS19; TADG14
NCBI Protein Information
kallikrein-8
UniProt Protein Name
Kallikrein-8
UniProt Gene Name
KLK8
UniProt Synonym Gene Names
NRPN; PRSS19; TADG14; hK8; NP
UniProt Entry Name
KLK8_HUMAN
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Product Notes
The Human KLK8 klk8 (Catalog #AAA331947) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331947 ELISA Kit recognizes Human KLK8. It is sometimes possible for the material contained within the vial of "Kallikrein 8 (KLK8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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