Human KLK5 ELISA Kit | KLK5 elisa kit

Human KLK5 PicoKine ELISA Kit

Cat. Num. AAA45262

• Gene Names: KLK5; SCTE; KLKL2; KLK-L2
• Reactivity: Human
• Synonyms: KLK5; N/A; Human KLK5 PicoKine ELISA Kit; Kallikrein-5; kallikrein-related peptidase 5; Kallikrein 5; Kallikrein-like protein 2; KLK L2; SCTE; Stratum corneum tryptic enzyme; KLK5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human KLK5
• Sequence Length: 293
• Samples: Cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva
• Detection Range: 78 pg/ml - 5,000 pg/ml
• Sensitivity: <10 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for KLK5 elisa kit

Background: Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Principle of the Assay: The Picokine™ Human KLK5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human KLK5 with a 96-well strip plate that is pre-coated with antibody specific for KLK5. The detection antibody is a biotinylated antibody specific for KLK5. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK5 with immunogen: Expression system for standard: NS0; Immunogen sequence: V23-S293. The kit is analytically validated with ready-to-use reagents. To measure Human KLK5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human KLK5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human KLK5 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 296434569
• NCBI GeneID: 25818
• UniProt Accession #: Q9Y337; Q53ZR3; Q9HBG8
• Molecular Weight: 32,019 Da
• NCBI Official Full Name: Kallikrein-5
• NCBI Official Synonym Full Names: kallikrein-related peptidase 5
• NCBI Official Symbol: KLK5
• NCBI Official Synonym Symbols: SCTE; KLKL2; KLK-L2
• NCBI Protein Information: kallikrein-5; kallikrein 5; kallikrein-like protein 2; stratum corneum tryptic enzyme
• UniProt Protein Name: Kallikrein-5
• UniProt Gene Name: KLK5
• UniProt Synonym Gene Names: SCTE; KLK-L2
• UniProt Entry Name: KLK5_HUMAN