Standard Curve (Sample)
(Fig.1. Human Lactoferrin (LTF) Standard Curve.)
Human Lactoferrin (LTF) ELISA Kit | LTF elisa kit
Human Lactoferrin (LTF) ELISA Kit
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human
Synonyms
Lactoferrin (LTF); N/A; Human Lactoferrin (LTF) ELISA Kit; LTF; GIG12; HLF2; LF; growth-inhibiting protein 12; lactoferrin; neutrophil lactoferrin; talalactoferrin; LTF elisa kit
Reactivity
Human
Specificity
Human Lactoferrin (LTF) ELISA Kit has high sensitivity and excellent specificity for detection of Human LTF. No significant cross-reactivity or interference between Human LTF and analogues was observed.
Sequence Length
475
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Lactoferrin (LTF) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Lactoferrin (LTF) Standard Curve.)
Related Product Information for LTF elisa kit
Description: This Human Lactoferrin (LTF) ELISA Kit employs a two-site sandwich ELISA to quantitate LTF in samples. An antibody specific for LTF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LTF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LTF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LTF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LTF, a globular multifunctional protein with antimicrobial activity (bacteriocide, fungicide), is part of the innate defense, mainly at mucoses. Lactoferrin is found in milk and many mucosal secretions such as tears and saliva. Lactoferrin is also present in secondary granules of PMN and also is secreted by some acinar cells. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin belongs to the transferrin family proteins (TF, melanotransferrin, ovotransferin, etc.). Its molecular mass is 80,000 u (80 kDa).Lactoferrin antimicrobial activity is due partly to its high affinity for Fe (ferric state). LF proteolysis produces lactoferricin, kaliocin-1 small peptides with antimicrobial activity.
Background: LTF, a globular multifunctional protein with antimicrobial activity (bacteriocide, fungicide), is part of the innate defense, mainly at mucoses. Lactoferrin is found in milk and many mucosal secretions such as tears and saliva. Lactoferrin is also present in secondary granules of PMN and also is secreted by some acinar cells. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin belongs to the transferrin family proteins (TF, melanotransferrin, ovotransferin, etc.). Its molecular mass is 80,000 u (80 kDa).Lactoferrin antimicrobial activity is due partly to its high affinity for Fe (ferric state). LF proteolysis produces lactoferricin, kaliocin-1 small peptides with antimicrobial activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
lactoferrin, partial
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
LTF
NCBI Official Synonym Symbols
LF; HLF2; GIG12; HEL110
NCBI Protein Information
lactotransferrin
UniProt Protein Name
Lactotransferrin
UniProt Gene Name
LTF
UniProt Synonym Gene Names
GIG12; LF; Lactoferrin; Lfcin-H
UniProt Entry Name
TRFL_HUMAN
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Product Notes
The Human LTF ltf (Catalog #AAA331912) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331912 ELISA Kit recognizes Human LTF. It is sometimes possible for the material contained within the vial of "Lactoferrin (LTF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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