Standard Curve (Sample)
(Fig.1. Human Leucine-rich PPR motif-containing protein, mitochondrial (LRPPRC) Standard Curve.)
Human Leucine-Rich PPR Motif-Containing Protein, Mitochondrial (LRPPRC) ELISA Kit | LRPPRC elisa kit
Human Leucine-Rich PPR Motif-Containing Protein, Mitochondrial (LRPPRC) ELISA Kit
Gene Names
LRPPRC; LSFC; GP130; LRP130; CLONE-23970
Reactivity
Human
Synonyms
Leucine-Rich PPR Motif-Containing Protein, Mitochondrial (LRPPRC); N/A; Human Leucine-Rich PPR Motif-Containing Protein, Mitochondrial (LRPPRC) ELISA Kit; LRPPRC; CLONE-23970; GP130; LRP130; LSFC; leucine-rich PPR motif-containing protein; LRPPRC elisa kit
Reactivity
Human
Specificity
Human Leucine-rich PPR motif-containing protein, mitochondrial (LRPPRC) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRPPRC. No significant cross-reactivity or interference between Human LRPPRC and analogues was observed.
Sequence Length
1394
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Leucine-rich PPR motif-containing protein, mitochondrial (LRPPRC) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Leucine-rich PPR motif-containing protein, mitochondrial (LRPPRC) Standard Curve.)
Related Product Information for LRPPRC elisa kit
Description: This Human Leucine-rich PPR motif-containing protein, mitochondrial (LRPPRC) ELISA Kit employs a two-site sandwich ELISA to quantitate LRPPRC in samples. An antibody specific for LRPPRC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRPPRC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRPPRC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRPPRC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LRPPRC contains 167 leucine residues that are dispersed every 15 to 19 amino acids throughout the protein, accounting for about 13% of the sequence. The N-terminal half of LRPPRC is cysteine rich. LRPPRC has 4 potential N-linked glycosylation sites, a sequence that shares homology with the ATP-binding site of ATP-dependent kinases, and a protein kinase C phosphorylation site. It has no secretory signal sequence or hydrophobic domains. Northern blot analysis of HepG2 cells revealed expression of a major 4.8-kb transcript and minor transcripts of 5.2 and 7.0 kb. Western blot analysis of transiently transfected COS-1 cells revealed a doublet with an apparent molecular mass of about 130 kD.
Background: LRPPRC contains 167 leucine residues that are dispersed every 15 to 19 amino acids throughout the protein, accounting for about 13% of the sequence. The N-terminal half of LRPPRC is cysteine rich. LRPPRC has 4 potential N-linked glycosylation sites, a sequence that shares homology with the ATP-binding site of ATP-dependent kinases, and a protein kinase C phosphorylation site. It has no secretory signal sequence or hydrophobic domains. Northern blot analysis of HepG2 cells revealed expression of a major 4.8-kb transcript and minor transcripts of 5.2 and 7.0 kb. Western blot analysis of transiently transfected COS-1 cells revealed a doublet with an apparent molecular mass of about 130 kD.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Leucine-rich PPR motif-containing protein, mitochondrial
NCBI Official Synonym Full Names
leucine rich pentatricopeptide repeat containing
NCBI Official Symbol
LRPPRC
NCBI Official Synonym Symbols
LSFC; GP130; LRP130; CLONE-23970
NCBI Protein Information
leucine-rich PPR motif-containing protein, mitochondrial
UniProt Protein Name
Leucine-rich PPR motif-containing protein, mitochondrial
UniProt Gene Name
LRPPRC
UniProt Synonym Gene Names
LRP130; LRP 130
UniProt Entry Name
LPPRC_HUMAN
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Product Notes
The Human LRPPRC lrpprc (Catalog #AAA331926) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331926 ELISA Kit recognizes Human LRPPRC. It is sometimes possible for the material contained within the vial of "Leucine-Rich PPR Motif-Containing Protein, Mitochondrial (LRPPRC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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