Standard Curve (Sample)
(Fig.1. Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) Standard Curve.)
Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit | LPCAT1 elisa kit
Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit
Gene Names
LPCAT1; AYTL2; lpcat; AGPAT9; PFAAP3; AGPAT10; LPCAT-1; lysoPAFAT
Reactivity
Human
Synonyms
Lysophosphatidylcholine Acyltransferase 1 (LPCAT1); N/A; Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit; LPCAT1; AYTL2; FLJ12443; FLJ41609; PFAAP3; lpcat; LPC acyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 1; acyltransferase like 2; phosphonoformate immuno-associated protein 3; regulated by phos; LPCAT1 elisa kit
Reactivity
Human
Specificity
Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT1. No significant cross-reactivity or interference between Human LPCAT1 and analogues was observed.
Sequence Length
534
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) Standard Curve.)
Related Product Information for LPCAT1 elisa kit
Description: This Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT1 in samples. An antibody specific for LPCAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPCAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lysophosphatidylcholine (LPC) acyltransferase (LPCAT) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis. deduced 534-amino acid mouse and human proteins contain 3 transmembrane domains, several motifs conserved in members of the LPCAT family, a putative EF hand domain, and a C-terminal endoplasmic reticulum (ER) retention signal. They share about 88% amino acid identity. Real-time RT-PCR of mouse tissues showed that Lpcat1 was expressed at highest levels in lung, followed by spleen and brain. Other tissues had low Lpcat1 levels. Confocal microscopy detected epitope-tagged Lpcat1 mainly in the ER and Golgi of transfected cells.
Background: Lysophosphatidylcholine (LPC) acyltransferase (LPCAT) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis. deduced 534-amino acid mouse and human proteins contain 3 transmembrane domains, several motifs conserved in members of the LPCAT family, a putative EF hand domain, and a C-terminal endoplasmic reticulum (ER) retention signal. They share about 88% amino acid identity. Real-time RT-PCR of mouse tissues showed that Lpcat1 was expressed at highest levels in lung, followed by spleen and brain. Other tissues had low Lpcat1 levels. Confocal microscopy detected epitope-tagged Lpcat1 mainly in the ER and Golgi of transfected cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lysophosphatidylcholine acyltransferase 1
NCBI Official Synonym Full Names
lysophosphatidylcholine acyltransferase 1
NCBI Official Symbol
LPCAT1
NCBI Official Synonym Symbols
AYTL2; lpcat; AGPAT9; PFAAP3; AGPAT10; LPCAT-1; lysoPAFAT
NCBI Protein Information
lysophosphatidylcholine acyltransferase 1
UniProt Protein Name
Lysophosphatidylcholine acyltransferase 1
UniProt Gene Name
LPCAT1
UniProt Synonym Gene Names
AYTL2; PFAAP3; LPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT
UniProt Entry Name
PCAT1_HUMAN
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Product Notes
The Human LPCAT1 lpcat1 (Catalog #AAA331939) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331939 ELISA Kit recognizes Human LPCAT1. It is sometimes possible for the material contained within the vial of "Lysophosphatidylcholine Acyltransferase 1 (LPCAT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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