Standard Curve (Sample)
(Fig.1. Human Lysophospholipid acyltransferase 5 (LPCAT3) Standard Curve.)
Human Lysophospholipid Acyltransferase 5 (LPCAT3) ELISA Kit | LPCAT3 elisa kit
Human Lysophospholipid Acyltransferase 5 (LPCAT3) ELISA Kit
Gene Names
LPCAT3; C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5
Reactivity
Human
Synonyms
Lysophospholipid Acyltransferase 5 (LPCAT3); N/A; Human Lysophospholipid Acyltransferase 5 (LPCAT3) ELISA Kit; LPCAT3; C3F; MBOAT5; OACT5; nessy; O-acyltransferase (membrane bound) domain containing 5; membrane bound O-acyltransferase domain containing 5; putative protein similar to nessy; LPCAT3 elisa kit
Reactivity
Human
Specificity
Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT3. No significant cross-reactivity or interference between Human LPCAT3 and analogues was observed.
Sequence Length
487
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Lysophospholipid acyltransferase 5 (LPCAT3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Lysophospholipid acyltransferase 5 (LPCAT3) Standard Curve.)
Related Product Information for LPCAT3 elisa kit
Description: This Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT3 in samples. An antibody specific for LPCAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPCAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 487-amino acid human and mouse MBOAT5 proteins contain 11 predicted transmembrane domains and share 88% amino acid identity. Human MBOAT5 shares 35% amino acid identity with Drosophila nessy.The deduced protein contains 10 putative transmembrane domains and a C-terminal endoplasmic reticulum (ER) retention motif (KKxx). RT-PCR detected ubiquitous Lpcat3 expression, with highest level in testis. Lpcat3 colocalized with an ER marker protein in transfected Chinese hamster ovary cells. Knockdown of Lpcat3 in mouse melanoma cells with small interfering RNA significantly reduced cellular acyltransferase activity toward endogenous lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.
Background: The deduced 487-amino acid human and mouse MBOAT5 proteins contain 11 predicted transmembrane domains and share 88% amino acid identity. Human MBOAT5 shares 35% amino acid identity with Drosophila nessy.The deduced protein contains 10 putative transmembrane domains and a C-terminal endoplasmic reticulum (ER) retention motif (KKxx). RT-PCR detected ubiquitous Lpcat3 expression, with highest level in testis. Lpcat3 colocalized with an ER marker protein in transfected Chinese hamster ovary cells. Knockdown of Lpcat3 in mouse melanoma cells with small interfering RNA significantly reduced cellular acyltransferase activity toward endogenous lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lysophospholipid acyltransferase 5
NCBI Official Synonym Full Names
lysophosphatidylcholine acyltransferase 3
NCBI Official Symbol
LPCAT3
NCBI Official Synonym Symbols
C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5
NCBI Protein Information
lysophospholipid acyltransferase 5
UniProt Protein Name
Lysophospholipid acyltransferase 5
UniProt Gene Name
LPCAT3
UniProt Synonym Gene Names
MBOAT5; OACT5; LPLAT 5; LPCAT; Lyso-PC acyltransferase; Lyso-PC acyltransferase 3; LPSAT; Lyso-PS acyltransferase; O-acyltransferase domain-containing protein 5
UniProt Entry Name
MBOA5_HUMAN
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Product Notes
The Human LPCAT3 lpcat3 (Catalog #AAA331937) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331937 ELISA Kit recognizes Human LPCAT3. It is sometimes possible for the material contained within the vial of "Lysophospholipid Acyltransferase 5 (LPCAT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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