Standard Curve (Sample)
(Fig.1. Human Lysosomal Pro-X carboxypeptidase (PRCP) Standard Curve.)
Human Lysosomal Pro-X Carboxypeptidase (PRCP) ELISA Kit | PRCP elisa kit
Human Lysosomal Pro-X Carboxypeptidase (PRCP) ELISA Kit
Gene Names
PRCP; PCP; HUMPCP
Reactivity
Human
Synonyms
Lysosomal Pro-X Carboxypeptidase (PRCP); N/A; Human Lysosomal Pro-X Carboxypeptidase (PRCP) ELISA Kit; PRCP; HUMPCP; MGC2202; PCP; angiotensinase C; lysosomal Pro-x carboxypeptidase; lysosomal carboxypeptidase C; proline carboxypeptidase; prolylcarboxypeptidase; prolylcarboxypeptidase isoform 1 preproprotein; PRCP elisa kit
Reactivity
Human
Specificity
Human Lysosomal Pro-X carboxypeptidase (PRCP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRCP. No significant cross-reactivity or interference between Human PRCP and analogues was observed.
Sequence Length
496
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Lysosomal Pro-X carboxypeptidase (PRCP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Lysosomal Pro-X carboxypeptidase (PRCP) Standard Curve.)
Related Product Information for PRCP elisa kit
Description: This Human Lysosomal Pro-X carboxypeptidase (PRCP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRCP in samples. An antibody specific for PRCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRCP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lysosomal Pro-X carboxypeptidase is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Background: Lysosomal Pro-X carboxypeptidase is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Lysosomal Pro-X carboxypeptidase
NCBI Official Synonym Full Names
prolylcarboxypeptidase
NCBI Official Symbol
PRCP
NCBI Official Synonym Symbols
PCP; HUMPCP
NCBI Protein Information
lysosomal Pro-X carboxypeptidase
UniProt Protein Name
Lysosomal Pro-X carboxypeptidase
UniProt Gene Name
PRCP
UniProt Synonym Gene Names
PCP; PRCP
UniProt Entry Name
PCP_HUMAN
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Product Notes
The Human PRCP prcp (Catalog #AAA331366) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331366 ELISA Kit recognizes Human PRCP. It is sometimes possible for the material contained within the vial of "Lysosomal Pro-X Carboxypeptidase (PRCP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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