Standard Curve (Sample)
Human Malectin (MLEC) ELISA Kit | MLEC elisa kit
Human Malectin (MLEC) ELISA Kit
Gene Names
MLEC; KIAA0152
Reactivity
Human
Synonyms
Malectin (MLEC); N/A; Human Malectin (MLEC) ELISA Kit; MLEC; KIAA0152; MLEC elisa kit
Reactivity
Human
Specificity
Human Malectin (MLEC) ELISA Kit has high sensitivity and excellent specificity for detection of Human MLEC. No significant cross-reactivity or interference between Human MLEC and analogues was observed.
Sequence Length
292
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
50 pg/ml - 800 pg/ml
Sensitivity
< 5pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MLEC elisa kit
Background: MLEC encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. This protein has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. This protein has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants.
Principle of the Assay: Human Malectin (MLEC) ELISA Kit employs a two-site sandwich ELISA to quantitate MLEC in samples. An antibody specific for MLEC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MLEC present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MLEC detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MLEC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Malectin (MLEC) ELISA Kit employs a two-site sandwich ELISA to quantitate MLEC in samples. An antibody specific for MLEC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MLEC present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MLEC detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MLEC bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
malectin isoform1
NCBI Official Synonym Full Names
malectin
NCBI Official Symbol
MLEC
NCBI Official Synonym Symbols
KIAA0152
NCBI Protein Information
malectin
UniProt Protein Name
Malectin
UniProt Gene Name
MLEC
UniProt Synonym Gene Names
KIAA0152
UniProt Entry Name
MLEC_HUMAN
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Product Notes
The Human MLEC mlec (Catalog #AAA331755) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331755 ELISA Kit recognizes Human MLEC. It is sometimes possible for the material contained within the vial of "Malectin (MLEC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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