Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit | SERPINB5 elisa kit

Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit

Cat. Num. AAA137157

• Gene Names: SERPINB5; PI5; maspin
• Reactivity: Human
• Synonyms: Mammary Serine Protease Inhibitor (Maspin); N/A; Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit; SERPINB5; PI5; Protease Inhibitor 5; Serpin Peptidase Inhibitor Clade B Member 5; SERPINB5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mammary Serine Protease Inhibitor (Maspin).; No significant cross-reactivity or interference between Mammary Serine Protease Inhibitor (Maspin) and analogues was observed.
• Sequence Length: 375
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and other biological fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.233ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mammary Serine Protease Inhibitor (Maspin) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mammary Serine Protease Inhibitor (Maspin) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for SERPINB5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mammary Serine Protease Inhibitor (Maspin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mammary Serine Protease Inhibitor (Maspin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mammary Serine Protease Inhibitor (Maspin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mammary Serine Protease Inhibitor (Maspin) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SERPINB5 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 453369
• NCBI GeneID: 5268
• UniProt Accession #: P36952; Q6N0B4; Q8WW89; B2R6Y4
• Molecular Weight: 25,763 Da
• NCBI Official Full Name: maspin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade B (ovalbumin), member 5
• NCBI Official Symbol: SERPINB5
• NCBI Official Synonym Symbols: PI5; maspin
• NCBI Protein Information: serpin B5
• UniProt Protein Name: Serpin B5
• UniProt Gene Name: SERPINB5
• UniProt Synonym Gene Names: PI5; PI-5
• UniProt Entry Name: SPB5_HUMAN