Human Mannose Binding Lectin (MBL) ELISA Kit | MBL elisa kit
Human Anti-Mannose Binding Lectin Antibody (Anti-MBL) ELISA Kit
Gene Names
MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
Reactivity
Human
Applications
ELISA
Synonyms
Mannose Binding Lectin (MBL); N/A; Human Anti-Mannose Binding Lectin Antibody (Anti-MBL) ELISA Kit; Mannose Binding Lectin; MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect); MBL elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-Mannose Binding Lectin Antibody (Anti-MBL).
No significant cross-reactivity or interference between Anti-Mannose Binding Lectin Antibody (Anti-MBL) and analogues was observed.
No significant cross-reactivity or interference between Anti-Mannose Binding Lectin Antibody (Anti-MBL) and analogues was observed.
Sequence Length
248
Assay Type
Competitive Inhibition
Samples
Serum, plasma and other biological fluids
Detection Range
6.25-400ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 2.45ng/mL
Applicable Applications for MBL elisa kit
ELISA
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Mannose Binding Lectin Antibody (Anti-MBL) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Mannose Binding Lectin Antibody (Anti-MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mannose-binding protein C
NCBI Official Synonym Full Names
mannose binding lectin 2
NCBI Official Symbol
MBL2
NCBI Official Synonym Symbols
MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
NCBI Protein Information
mannose-binding protein C
UniProt Protein Name
Mannose-binding protein C
UniProt Gene Name
MBL2
UniProt Synonym Gene Names
COLEC1; MBL; MBP-C
UniProt Entry Name
MBL2_HUMAN
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Product Notes
The Human MBL mbl2 (Catalog #AAA188371) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA188371 ELISA Kit recognizes Human MBL. AAA Biotech's Mannose Binding Lectin (MBL) can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the MBL mbl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mannose Binding Lectin (MBL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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