Standard Curve (Sample)
(Fig.1. Human MAP kinase-activated protein kinase 5 (MAPKAPK5) Standard Curve.)
Human MAP Kinase-Activated Protein Kinase 5 (MAPKAPK5) ELISA Kit | MAPKAPK5 elisa kit
Human MAP Kinase-Activated Protein Kinase 5 (MAPKAPK5) ELISA Kit
Gene Names
MAPKAPK5; MK5; MK-5; PRAK; MAPKAP-K5
Reactivity
Human
Synonyms
MAP Kinase-Activated Protein Kinase 5 (MAPKAPK5); N/A; Human MAP Kinase-Activated Protein Kinase 5 (MAPKAPK5) ELISA Kit; MAPKAPK5; PRAK; MAP kinase-activated protein kinase 5; p38-regulated/activated protein kinase; MAPKAPK5 elisa kit
Reactivity
Human
Specificity
Human MAP kinase-activated protein kinase 5 (MAPKAPK5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPKAPK5. No significant cross-reactivity or interference between Human MAPKAPK5 and analogues was observed.
Sequence Length
471
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human MAP kinase-activated protein kinase 5 (MAPKAPK5) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human MAP kinase-activated protein kinase 5 (MAPKAPK5) Standard Curve.)
Related Product Information for MAPKAPK5 elisa kit
Description: This Human MAP kinase-activated protein kinase 5 (MAPKAPK5) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAPK5 in samples. An antibody specific for MAPKAPK5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPKAPK5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAPK5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAPK5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAP kinase-activated protein kinase 5 is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.MAPKAPK5 encodes a predicted 471-amino acid protein that shares 20 to 30% sequence identity with RSK, MNK, and MAPKAPK kinases. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases.
Background: MAP kinase-activated protein kinase 5 is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.MAPKAPK5 encodes a predicted 471-amino acid protein that shares 20 to 30% sequence identity with RSK, MNK, and MAPKAPK kinases. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
MAP kinase-activated protein kinase 5 isoform 1
NCBI Official Synonym Full Names
MAPK activated protein kinase 5
NCBI Official Symbol
MAPKAPK5
NCBI Official Synonym Symbols
MK5; MK-5; PRAK; MAPKAP-K5
NCBI Protein Information
MAP kinase-activated protein kinase 5
UniProt Protein Name
MAP kinase-activated protein kinase 5
UniProt Gene Name
MAPKAPK5
UniProt Synonym Gene Names
PRAK; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; PRAK
UniProt Entry Name
MAPK5_HUMAN
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Product Notes
The Human MAPKAPK5 mapkapk5 (Catalog #AAA331876) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331876 ELISA Kit recognizes Human MAPKAPK5. It is sometimes possible for the material contained within the vial of "MAP Kinase-Activated Protein Kinase 5 (MAPKAPK5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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