Standard Curve (Sample)
(Fig.1. Human Maternal embryonic leucine zipper kinase (MELK) Standard Curve.)
Human Maternal Embryonic Leucine Zipper Kinase (MELK) ELISA Kit | MELK elisa kit
Human Maternal Embryonic Leucine Zipper Kinase (MELK) ELISA Kit
Gene Names
MELK; HPK38
Reactivity
Human
Synonyms
Maternal Embryonic Leucine Zipper Kinase (MELK); N/A; Human Maternal Embryonic Leucine Zipper Kinase (MELK) ELISA Kit; MELK; RP11-8N6.1; HPK38; KIAA0175; pEg3 kinase; MELK elisa kit
Reactivity
Human
Specificity
Human Maternal embryonic leucine zipper kinase (MELK) ELISA Kit has high sensitivity and excellent specificity for detection of Human MELK. No significant cross-reactivity or interference between Human MELK and analogues was observed.
Sequence Length
610
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Maternal embryonic leucine zipper kinase (MELK) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Maternal embryonic leucine zipper kinase (MELK) Standard Curve.)
Related Product Information for MELK elisa kit
Description: This Human Maternal embryonic leucine zipper kinase (MELK) ELISA Kit employs a two-site sandwich ELISA to quantitate MELK in samples. An antibody specific for MELK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MELK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MELK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MELK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MELK (Maternal Embryonic Leucine Zipper Kinase) is a Protein Coding gene. Among its related pathways are Neuroscience. GO annotations related to this gene include calcium ion binding and protein kinase activity. An important paralog of this gene is PRKAA1. Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
Background: MELK (Maternal Embryonic Leucine Zipper Kinase) is a Protein Coding gene. Among its related pathways are Neuroscience. GO annotations related to this gene include calcium ion binding and protein kinase activity. An important paralog of this gene is PRKAA1. Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
maternal embryonic leucine zipper kinase isoform 2
NCBI Official Synonym Full Names
maternal embryonic leucine zipper kinase
NCBI Official Symbol
MELK
NCBI Official Synonym Symbols
HPK38
NCBI Protein Information
maternal embryonic leucine zipper kinase
UniProt Protein Name
Maternal embryonic leucine zipper kinase
UniProt Gene Name
MELK
UniProt Synonym Gene Names
KIAA0175; hMELK; pEg3 kinase; hPK38
UniProt Entry Name
MELK_HUMAN
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Product Notes
The Human MELK melk (Catalog #AAA331807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331807 ELISA Kit recognizes Human MELK. It is sometimes possible for the material contained within the vial of "Maternal Embryonic Leucine Zipper Kinase (MELK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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