Reactivity
Human
Specificity
Human Matrilin-3 (MATN3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MATN3. No significant cross-reactivity or interference between Human MATN3 and analogues was observed.
Sequence Length
486
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
25 pg/ml - 400 pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MATN3 elisa kit
Background: Matrin 3(MATR3) is localized in the nuclear matrix. It may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. Two transcript variants encoding the same protein have been identified for this gene.The deduced 845-amino acid protein has a calculated molecular mass of approximately 95 kD. Its primary structure consists of 33% charged residues and is generally hydrophilic. Like the lamins, matrin-3 has a positively charged N terminus that contains a large number of amino acids with free hydroxyl groups. A highly acidic domain near the C terminus, in which 32% of the amino acids are acidic, is a characteristic found in other nuclear proteins.
Principle of the Assay: Human Matrilin-3 (MATN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN3 in samples. An antibody specific for MATN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MATN3 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MATN3 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MATN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Matrilin-3 (MATN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN3 in samples. An antibody specific for MATN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MATN3 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MATN3 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MATN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
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Product Notes
The Human MATN3 matn3 (Catalog #AAA331859) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331859 ELISA Kit recognizes Human MATN3. It is sometimes possible for the material contained within the vial of "Matrilin-3 (MATN3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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