Human MCP-1 ELISA Kit | MCP-1 elisa kit

Human MCP-1 PicoKine Quick ELISA Kit

Cat. Num. AAA128055

• Gene Names: CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Reactivity: Human
• Applications: ELISA
• Synonyms: MCP-1; N/A; Human MCP-1 PicoKine Quick ELISA Kit; C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Small-inducible cytokine A2; CCL2; MCP1, SCYA2; MCP-1 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine
• Assay Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <1pg/ml
• Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: Q24-T99
• Anticoagulant: heparin, EDTA or citrate
• Cross Reactivity: There is cross-reactivity with human Eotaxin, MCP-3<1%.

• Applicable Applications for MCP-1 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.025	0.071	0.154	0.244	0.466	1.052	1.298	1.892

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	31	159	477	30	148	481
  Standard deviation	1.55	8.74	32.91	1.98	9.76	37.51
  CV (%)	5%	5.5%	6.9%	6.6%	6.6%	7.8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	31	33	37	35	34	2.23	6.5%
  Sample 2	159	139	156	158	153	8.15	5.3%
  Sample 3	477	421	423	456	444	23.46	5.2%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human MCP-1 PicoKine ELISA Kit standard curve)

Related Product Information for MCP-1 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for MCP-1 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 545465
• NCBI GeneID: 6347
• UniProt Accession #: P13500; Q9UDF3; B2R4V3
• Molecular Weight: 11,025 Da
• NCBI Official Full Name: MCP-1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: CCL2
• NCBI Official Synonym Symbols: HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• NCBI Protein Information: C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine
• UniProt Protein Name: C-C motif chemokine 2
• UniProt Gene Name: CCL2
• UniProt Synonym Gene Names: MCP1; SCYA2; MCAF; MCP-1
• UniProt Entry Name: CCL2_HUMAN