Human Measles Virus IgG ELISA Kit
Measles Virus IgG ELISA Kit
Reactivity
Human
Synonyms
Measles Virus IgG; N/A; Measles Virus IgG ELISA Kit; Measles Virus IgG elisa kit
Reactivity
Human
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is 100% (95% confidence interval: 90.0% - 100%).
Samples
Human serum or plasma (citrate, heparin)
Preparation and Storage
2 - 8 degree C
Related Product Information for Measles Virus IgG elisa kit
Intended Uses: The Measles Virus IgG ELISA is intended for the qualitative determination of IgG class antibodies against Measles Virus in human serum or plasma (citrate, heparin).
Principle of the Assay: The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.
Background/Introduction: Measles or morbilli virus belongs to the RNA viruses of the family Paramyxoviridae. The virions are spherical particles of 150-250 nm in diameter consisting of the ribonucleoprotein with helical symmetry and an envelope with spikes containing the strain-specific and hemagglutinating antigens. Morbilli vi-ruses have no neuraminidase activity. Measles is a classic childhood disease. The virus is endemic: at the age of 20 about 90% of the population has had immunological experience with it. Newborns are protected by maternal antibodies for the first 3-4 months of life; the active disease leaves lifelong im-munity. The measles virus has a contagiousity index of about 96%, is worldwide distributed, and can be serious. Bacterial superinfection was a serious threat in the preantibiotic era, but the prognosis of un-complicated measles is now good. CNS complications such as encephalomyelitis (0.1%) which may occur after the acute phase of measles infection subsides, however still have a high mortality (10%). Prognosis of recovery in these patients is poor. Between 10-30% of all cases are fatal; 20-50% develop significant damages. Subacute sclerosing panencephalitis (SSPE) is a rare (1:1000) degenerative dis-ease of the CNS which is thought to be a slow virus infection.
Principle of the Assay: The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.
Background/Introduction: Measles or morbilli virus belongs to the RNA viruses of the family Paramyxoviridae. The virions are spherical particles of 150-250 nm in diameter consisting of the ribonucleoprotein with helical symmetry and an envelope with spikes containing the strain-specific and hemagglutinating antigens. Morbilli vi-ruses have no neuraminidase activity. Measles is a classic childhood disease. The virus is endemic: at the age of 20 about 90% of the population has had immunological experience with it. Newborns are protected by maternal antibodies for the first 3-4 months of life; the active disease leaves lifelong im-munity. The measles virus has a contagiousity index of about 96%, is worldwide distributed, and can be serious. Bacterial superinfection was a serious threat in the preantibiotic era, but the prognosis of un-complicated measles is now good. CNS complications such as encephalomyelitis (0.1%) which may occur after the acute phase of measles infection subsides, however still have a high mortality (10%). Prognosis of recovery in these patients is poor. Between 10-30% of all cases are fatal; 20-50% develop significant damages. Subacute sclerosing panencephalitis (SSPE) is a rare (1:1000) degenerative dis-ease of the CNS which is thought to be a slow virus infection.
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Product Notes
The Human Measles Virus IgG (Catalog #AAA72695) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA72695 ELISA Kit recognizes Human Measles Virus IgG. It is sometimes possible for the material contained within the vial of "Measles Virus IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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