Standard Curve (Sample)
(Fig.1. Human Mesoderm posterior protein 2 (MESP2) Standard Curve.)
Human Mesoderm Posterior Protein 2 (MESP2) ELISA Kit | MESP2 elisa kit
Human Mesoderm Posterior Protein 2 (MESP2) ELISA Kit
Gene Names
MESP2; SCDO2; bHLHc6
Reactivity
Human
Synonyms
Mesoderm Posterior Protein 2 (MESP2); N/A; Human Mesoderm Posterior Protein 2 (MESP2) ELISA Kit; MESP2; SCDO2; bHLHc6; mesoderm posterior 2 homolog; MESP2 elisa kit
Reactivity
Human
Specificity
Human Mesoderm posterior protein 2 (MESP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MESP2. No significant cross-reactivity or interference between Human MESP2 and analogues was observed.
Sequence Length
397
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Mesoderm posterior protein 2 (MESP2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Mesoderm posterior protein 2 (MESP2) Standard Curve.)
Related Product Information for MESP2 elisa kit
Description: This Human Mesoderm posterior protein 2 (MESP2) ELISA Kit employs a two-site sandwich ELISA to quantitate MESP2 in samples. An antibody specific for MESP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MESP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MESP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MESP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MESP2 encodes a member of the bHLH family of transcription factors and plays a key role in defining the rostrocaudal patterning of somites via interactions with multiple Notch signaling pathways. MESP2 is expressed in the anterior presomitic mesoderm and is downregulated immediately after the formation of segmented somites. MESP2 also plays a role in the formation of epithelial somitic mesoderm and cardiac mesoderm. Mutations in the MESP2 gene cause autosomal recessive spondylocostal dystosis 2 (SCD02).
Background: MESP2 encodes a member of the bHLH family of transcription factors and plays a key role in defining the rostrocaudal patterning of somites via interactions with multiple Notch signaling pathways. MESP2 is expressed in the anterior presomitic mesoderm and is downregulated immediately after the formation of segmented somites. MESP2 also plays a role in the formation of epithelial somitic mesoderm and cardiac mesoderm. Mutations in the MESP2 gene cause autosomal recessive spondylocostal dystosis 2 (SCD02).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mesoderm posterior protein 2
NCBI Official Synonym Full Names
mesoderm posterior bHLH transcription factor 2
NCBI Official Symbol
MESP2
NCBI Official Synonym Symbols
SCDO2; bHLHc6
NCBI Protein Information
mesoderm posterior protein 2
UniProt Protein Name
Mesoderm posterior protein 2
UniProt Gene Name
MESP2
UniProt Synonym Gene Names
BHLHC6; SCDO2; bHLHc6
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Product Notes
The Human MESP2 mesp2 (Catalog #AAA331796) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331796 ELISA Kit recognizes Human MESP2. It is sometimes possible for the material contained within the vial of "Mesoderm Posterior Protein 2 (MESP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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