Standard Curve (Sample)
Human met proto-oncogene (hepatocyte growth factor receptor) ELISA Kit | MET elisa kit
Human hepatocyte growth factor receptor, c-MET/HGFR ELISA Kit
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met
Reactivity
Human
Synonyms
met proto-oncogene (hepatocyte growth factor receptor); N/A; Human hepatocyte growth factor receptor, c-MET/HGFR ELISA Kit; Human hepatocyte growth factor receptor (c-MET/HGFR) ELISA Kit; AUTS9; HGFR; RCCP2; c-Met; HGF receptor; SF receptor; met proto-oncogene; met proto-oncogene tyrosine kinase; oncogene MET; scatter factor receptor; MET elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human c-MET. No significant cross-reactivity or interference between human c-MET and analogues was observed.
Sequence Length
1390
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
1.56 ng/ml-100 ng/ml.
Sensitivity
The minimum detectable dose of human c-MET is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MET elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for c-MET has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any c-MET present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for c-MET is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of c-MET bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
155,541 Da
NCBI Official Full Name
hepatocyte growth factor receptor isoform b
NCBI Official Synonym Full Names
MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol
MET
NCBI Official Synonym Symbols
HGFR; AUTS9; RCCP2; c-Met
NCBI Protein Information
hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Gene Name
MET
UniProt Synonym Gene Names
HGF receptor; SF receptor
UniProt Entry Name
MET_HUMAN
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Product Notes
The Human MET met (Catalog #AAA80800) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80800 ELISA Kit recognizes Human MET. It is sometimes possible for the material contained within the vial of "met proto-oncogene (hepatocyte growth factor receptor), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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