Human Metastasis Associated Protein 2 (MTA2) ELISA Kit | MTA2 elisa kit

Human Metastasis Associated Protein 2 (MTA2) ELISA Kit

Cat. Num. AAA186886

• Gene Names: MTA2; PID; MTA1L1
• Reactivity: Human
• Synonyms: Metastasis Associated Protein 2 (MTA2); N/A; Human Metastasis Associated Protein 2 (MTA2) ELISA Kit; MTA1L1; PID; Metastasis-associated 1-like 1; p53 target protein in deacetylase complex; Metastasis-associated protein MTA2; MTA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MTA2. No significant cross-reactivity or interference between MTA2 and analogues was observed.
• Sequence Length: 495
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 31pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MTA2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MTA2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for MTA2 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MTA2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MTA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MTA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MTA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MTA2 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

References

Serum proteomic profiling reveals MTA2 and AGO2 as potential prognostic biomarkers associated with disease activity and adverse outcomes in multiple myeloma; PLoS One; 2022 Dec 1;17(12):e0278464.; PMID: 36454786; Dollapak Apipongrat; Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Bangkok, Pathumthani, Thailand.

NCBI and Uniprot Product Information

• NCBI GI #: 14141170
• NCBI GeneID: 9219
• NCBI Accession #: NP_004730.2
• NCBI GenBank Nucleotide #: NM_004739.3
• UniProt Accession #: O94776; Q68DB1; Q9UQB5
• Molecular Weight: 55,037 Da
• NCBI Official Full Name: metastasis-associated protein MTA2 isoform 1
• NCBI Official Synonym Full Names: metastasis associated 1 family member 2
• NCBI Official Symbol: MTA2
• NCBI Official Synonym Symbols: PID; MTA1L1
• NCBI Protein Information: metastasis-associated protein MTA2
• UniProt Protein Name: Metastasis-associated protein MTA2
• UniProt Gene Name: MTA2
• UniProt Synonym Gene Names: MTA1L1; PID; MTA1-L1 protein