Standard Curve (Sample)
Human Methemoglobin (MHB) ELISA Kit | MHB elisa kit
Human Methemoglobin (MHB) ELISA Kit
Reactivity
Human
Synonyms
Methemoglobin (MHB); Human Methemoglobin (MHB) ELISA Kit; MHB elisa kit
Reactivity
Human
Specificity
Human Methemoglobin (MHB) ELISA Kit has high sensitivity and excellent specificity for detection of Human MHB. No significant cross-reactivity or interference between Human MHB and analogues was observed.
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
2.5 ng/ml - 40 ng/ml
Sensitivity
< 0.1ng/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MHB elisa kit
Background: Methemoglobin is a form of the oxygen-carrying metalloprotein hemoglobin, in which the iron in the heme group is in the Fe3+ (ferric) state, not the Fe2+ (ferrous) of normal hemoglobin. Methemoglobin cannot bind oxygen, unlike oxyhemoglobin. It is bluish chocolate-brown in color. In human blood a trace amount of methemoglobin is normally produced spontaneously, but when present in excess the blood becomes abnormally dark bluish brown. The NADH-dependent enzyme methemoglobin reductase (diaphorase I) is responsible for converting methemoglobin back to hemoglobin.
Principle of the Assay: Human Methemoglobin (MHB) ELISA Kit employs a two-site sandwich ELISA to quantitate MHB in samples. An antibody specific for MHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MHB present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MHB detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Methemoglobin (MHB) ELISA Kit employs a two-site sandwich ELISA to quantitate MHB in samples. An antibody specific for MHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MHB present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MHB detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Product Notes
The Human MHB (Catalog #AAA31496) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA31496 ELISA Kit recognizes Human MHB. It is sometimes possible for the material contained within the vial of "Methemoglobin (MHB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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